K. pneumoniae strains were cultured overnight in LB broth. On the following day, 10 µl of culture was added to slides and allowed to air dry. The dried bacteria were then heat fixed to the slide (3 min at 56°C); 10 µl of India ink (Becton, Dickinson and Company, Sparks, MD) diluted 1:3 in PBS was added and wet mounted with a coverslip. Superfrost slides (Thermo Fisher Scientific, Waltham, MA) were viewed and imaged using an Axioplan2 phase-contrast microscope (Zeiss, Irvine, CA) with a SPOT diagnostic camera and software (SPOT Imagine Solutions, Sterling Heights, MI).
Axioplan2 phase contrast microscope
Manufactured by Zeiss
The Axioplan2 is a phase-contrast microscope manufactured by Zeiss. It is designed for high-contrast imaging of unstained, transparent specimens. The microscope utilizes phase-contrast optics to enhance the visibility of details in living cells and other transparent samples.
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Lab products found in correlation
2 protocols using axioplan2 phase contrast microscope
1
Capsule Visualization of Klebsiella pneumoniae
2
Measuring V. natriegens Cell Dimensions
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V. natriegens precultures were grown overnight from a single colony, inoculated 1:100 in LBv2 media, and grown at 37 °C with 200 rpm for 1.5 h. 1.5 µL of exponential phase cultures were immobilized on 2% low gelling agarose (Sigma) pads containing LBv2 media and analyzed using an Axioplan 2 phase contrast microscope (Zeiss) and a Plan Neofluar 100× objective (Zeiss). Extraction of cell length and width was performed using bacstalk55 (link).
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