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Haemotoxylin

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Haemotoxylin is a natural dye extracted from the logwood tree. It is a common stain used in histology and pathology laboratories to visualize cell nuclei in tissue samples. Haemotoxylin stains nuclei a blue-purple color, allowing for the identification and examination of cellular structures.

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Lab products found in correlation

Pannoramic 250, by 3DHISTECH (2 mentions) Neutral buffered formalin, by Merck Group (1 mentions) Sodium bicarbonate, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Magnesium sulphate, by Merck Group (1 mentions) Histoclear, by National Diagnostics (1 mentions)

3 protocols using haemotoxylin

1

Tumor tissue analysis in mice

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From mice euthanized at 24 h, 7 d, and 14 d, excised tumour tissues were fixed in 4% paraformaldehyde (cat. # 43368, Alfa Aesar) overnight at 4 °C or, if tumours reached a size >1 cm as in control mice, at room temperature to ensure complete fixation. Tissues were then washed 3 times in water and stored until further processing in 70% ethanol (cat. # 459836, Sigma-Aldrich). After embedding in paraffin (cat. # 39601006, Leica Biosystems), the tumours were cut into 10 μm slices and stained using Haemotoxylin (cat. # 26381–02, Electron Microscopy Sciences) and Eosin (cat. # HT110216, Sigma-Aldrich) (H&E) for general tissue staining, antibody against CD31 (Dianova, cat. # DIA-310, 2ug/ml) for endothelial cells and thus blood vessels, and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL(cat. # 11093070910, Sigma-Aldrich)) to detect apoptotic cells. Staining was performed by the MSK Cytology Core facility following established protocols. The tissue slices were scanned with a high-resolution digital slide scanner (Pannoramic 250, 3DHistech).
Haedicke K., Agemy L., Omar M., Berezhnoi A., Roberts S., Longo-Machado C., Skubal M., Nagar K., Hsu H.T., Kim K., Reiner T., Coleman J., Ntziachristos V., Scherz A, & Grimm J. (2020). High-resolution optoacoustic imaging of tissue responses to vascular-targeted therapies. Nature biomedical engineering, 4(3), 286-297.
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2

Endometrial Tissue Embedding and Sectioning

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Endometrial biopsies were removed from 10% neutral buffered formalin (Sigma-Aldrich) and immersed in 70% IMS for 24 h before overnight paraffin wax embedding in Surgipath cassettes (Leica Biosystems, Newcastle, UK). Embedded biopsies were sectioned at 3 μm, dewaxed by immersion in histoclear (National Diagnostics, Atlanta, GA, USA) and rehydrated by immersion in 100%, 90%, 80% and 75% ethanol (Sigma-Aldrich). Haemotoxylin (Leica Biosystems) was applied to the tissue sections for 10 min before immersion in Scott’s tap water (30 g magnesium sulphate (Sigma-Aldrich) and 2 g sodium bicarbonate (Sigma-Aldrich) in 3 L tap water) for 5 mins. Tissue sections were progressively dehydrated by a reversal of the ethanol immersion steps used previously and mounted with Surgipath mounting medium (Leica Biosystems). Slides were examined using an Olympus BX51 upright microscope (Leica Biosystems).
Kelly P., Barry-Reidy A., Brewer A., Meade K.G, & O’Farrelly C. (2020). Improved filtration method to isolate pure populations of primary bovine endometrial epithelial and stromal cells for immunological studies. Veterinary Research Communications, 44(1), 29-39.
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3

Tumor tissue analysis in mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
From mice euthanized at 24 h, 7 d, and 14 d, excised tumour tissues were fixed in 4% paraformaldehyde (cat. # 43368, Alfa Aesar) overnight at 4 °C or, if tumours reached a size >1 cm as in control mice, at room temperature to ensure complete fixation. Tissues were then washed 3 times in water and stored until further processing in 70% ethanol (cat. # 459836, Sigma-Aldrich). After embedding in paraffin (cat. # 39601006, Leica Biosystems), the tumours were cut into 10 μm slices and stained using Haemotoxylin (cat. # 26381–02, Electron Microscopy Sciences) and Eosin (cat. # HT110216, Sigma-Aldrich) (H&E) for general tissue staining, antibody against CD31 (Dianova, cat. # DIA-310, 2ug/ml) for endothelial cells and thus blood vessels, and terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL(cat. # 11093070910, Sigma-Aldrich)) to detect apoptotic cells. Staining was performed by the MSK Cytology Core facility following established protocols. The tissue slices were scanned with a high-resolution digital slide scanner (Pannoramic 250, 3DHistech).
Haedicke K., Agemy L., Omar M., Berezhnoi A., Roberts S., Longo-Machado C., Skubal M., Nagar K., Hsu H.T., Kim K., Reiner T., Coleman J., Ntziachristos V., Scherz A, & Grimm J. (2020). High-resolution optoacoustic imaging of tissue responses to vascular-targeted therapies. Nature biomedical engineering, 4(3), 286-297.
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