Miconazole nitrate salt

Acetone (SZBE082SV) of analytical grade was purchased from Sigma-Aldrich Co. (USA). Absolute ethanol (E/0650DF/15) was purchased from Fischer Chemicals (UK). Sterile deionized water was produced by a water purification system (Milli-Q Reagent Water System, MA, USA). Yeast extract and Bacto™ peptone were purchased from Lab M Ltd. (Lancashire, UK). Ammonium chloride, calcium chloride, cholesterol, ciprofloxacin hydrochloride, dextrose, dimethyl sulphoxide (DMSO, molecular biology grade), levamisole hydrochloride, magnesium sulfate, (±)-miconazole nitrate salt, rupintrivir, potassium dihydrogen phosphate, di–sodium hydrogen phosphate, sodium chloride, sodium hydroxide, and sucrose were all purchased from Sigma–Aldrich (MO, USA). MTS (3–[4,5 dimethylthiazol–2–yl]–5–[3–carboxymethyl–phenyl]–2–[4–sulphophenyl]–2H–tetrazolium, inner salt), was purchased from Promega, Leiden, The Netherlands.

HPLC-grade n-hexane, ethyl acetate, methanol, and dimethyl sulfoxide (DMSO, molecular biology grade) were purchased from Sigma–Aldrich Co. (MO, USA). Sterile deionized water was produced by a Milli–Q Reagent Water System (MA, USA). Bacto™ peptone and yeast extract were from Lab M Ltd. (Lancashire, UK). Ciprofloxacin (LOT: 105M4195V, antibacterial control), chloramphenicol (LOT: 015 K0562, antibacterial control only for EF due to their resistance to Ciprofloxacin) [37 (link)], (±)- miconazole nitrate salt (LOT: 085M4092V, antifungal control) and gossypol (LOT: 024M4030V, cytotoxic control) were all purchased from Sigma–Aldrich (MO, USA). For cell culture materials, fetal bovine serum (FBS), Dulbecco-Modified Eagle’s Medium-high glucose (DMEM), Hanks’s Balanced Salt Solution (HBSS), Phosphate-Buffered Saline (PBS), and penicillin-streptomycin solution (P/S) were from Sigma–Aldrich (MO, USA).