Cell proliferation was evaluated by the CCK-8 (Targetmol, USA) assay. The A2780 cells (Control and shLIMD2) were passaged to 96-well plates (1 × 103 cells/well) and cultured for the indicated time periods (24 h, 48 h, 72 h, 96 h, 120 h) before the addition of CCK-8 (5 mg/ml, 10 μL/wells) reagent. The absorbance of all the wells at 450 nm was detected with a Thermomax microplate reader (Molecular Devices, Sunnyvale, USA). Three biological replicates were performed.
Cck 8
Manufactured by Targetmol
Sourced in United States, China
CCK-8 is a colorimetric assay kit used to measure cell viability and cytotoxicity. It utilizes the water-soluble tetrazolium salt WST-8 to produce a water-soluble formazan dye upon reduction in the presence of an electron carrier. The amount of formazan dye generated is directly proportional to the number of living cells.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Agilent Technologies (2 mentions)
Thermomax microplate reader, by Molecular Devices (1 mentions)
Microplate analyzer, by Promega (1 mentions)
Varioskan, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Mqx200, by Agilent Technologies (1 mentions)
Pe annexin 5 apoptosis detection kit, by BD (1 mentions)
Apc brdu flow kit, by BD (1 mentions)
Imark microplate absorbance reader, by Bio-Rad (1 mentions)
96 well microtiter plate, by Corning (1 mentions)
Sb202190, by Merck Group (1 mentions)
Spectramax plus 384 microplate reader, by Molecular Devices (1 mentions)
Enspire 2300, by PerkinElmer (1 mentions)
N acetyl cysteine (nac), by MedChemExpress (1 mentions)
Vitamin c, by Merck Group (1 mentions)
Eclipse ti s fluorescence microscope, by Nikon (1 mentions)
Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions)
Dmi3000b, by Leica camera (1 mentions)
23 protocols using cck 8
1
Cell Proliferation Assay with CCK-8
2
Cell Proliferation Assay with Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in 96-well plates, incubated for 12 h and then replaced with complete DMEM/F12 medium containing different concentrations of inhibitors for 24 h. Cell proliferation was evaluated by CCK-8 (TargetMol, Shanghai, China) analysis according to the manufacturer’s instructions. The optical density (OD) was recorded at 450 nm.
3
Cell Viability Assay of AML Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AML cells (1 × 104 per well) were seeded in 100 μl medium in 96-well plates. The cell viability was determined by cell counting kit -8(CCK-8, TargetMol) after 24, 48 and 72 h of treatment with DMSO or ritanserin with specific concentration. Three replicates were presented and the results were expressed as the percentage of living cells compared with the control group. GraphPad Prism 8 was used for statistical analysis and image rendering.
4
Optimizing EV Concentration for Cell Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the optimal concentration of EVs to promote HK-2 and HUVEC proliferation, a cell counting kit 8 (CCK-8) assay was performed. In brief, cells were seeded in 96-well plates (2 × 103/well) and treated with different concentrations of EVs for 48 h. Then 10 μl CCK-8 (TargetMol, MA, USA) solution was added to each well and incubated at 37 °C for 2 h. The optical density (OD) values at 450 nm were read using a microplate analyzer (Promega, WI, USA).
5
Cytotoxicity and miRNA Delivery Assay of PCP
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PCP were synthesized according to the method we described previously [8 ]. To detect the cytotoxicity of PCP, about 7000 MC38 cells per well were seeded into 96 well plates and cultured in RPMI-1640 medium with 10% FBS (GIBCO, New Zealand) under a humid atmosphere containing 5% CO2 at 37 °C. When the confluence of cells was 70%, RPMI-1640 medium containing 10% FBS and different concentrations (0, 5, 10, 20, 50, 100 μg mL−1) of PCP were added into the plate. After 24 h, 10 μl of CCK8 (TargetMol, C0005) was added to each well. Then cell viability was measured by using Thermo Fisher Varioskan (Thermo) according to the manufacturer's instructions. And the cytotoxicity of PCP (50 μg mL−1) at different time points (0, 4, 8, 12, 24, 48 h) was also measured. For miRNA delivery ability test, NPs/cy3miRNA nanocomplexes self-assembled with PCP (80 μg mL−1) and cy3 fluorescently labeled miRNA365 (40 nM) were added into 293 T cells and MC38 cells culture medium, respectively. After 24 h of transfection, photos were collected by fluorescence microscope.
6
Cisplatin Cytotoxicity Evaluation in Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells (5 × 104/mL) were seeded into 96 well plates with 100uL per well. After 24 h, different concentrations (0, 0.25, 0.5, 1, 2, 4, 8, 16, 32 ug/mL) of cisplatin were added and incubated for 48 h. Cell viability was measured by cell counting kit-8 (CCK8, Targetmol). A complete medium containing 10% CCK8 was added into each well of plate, which was placed in a dark environment at 37℃ for 2–4 h. Then, a microplate reader (KHB ST-360, shanghai) was used to detect the absorbance (OD value) at 450 nm. Cells incubated with 10% CCK8 complete medium were set as control. The wells only containing 10% CCK8 complete medium were used as blank. Cell viability = (OD experiment - OD blank) / (OD control - OD blank).
7
Cell Cytotoxicity Assay with RPDPD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Seeded in a 96-well plate at a density of 2 × 104 cells/well, the original medium was removed after the cells adhered the next day, and the compound RPDPD (0 μmol/L, 3.125 μmol/L, 6.25 μmol/L, 12.5 μmol/L, 25 μmol/L, 50 μmol/L, and 100 μmol/L) medium was added and cultured for 24, 48, and 72 h, respectively. With DMSO as the control group, cisplatin treatment was used as a positive control, and normal hepatocytes were used for cytotoxicity experiments, and CCK8 (TargetMol, Shanghai, China) was incubated for 2 h. Also, the plate was evaluated in a luminescence microplate reader (BioTEK INC, Biotek MQX200) at 450 nm wavelength. The optical density (OD) values were obtained. The inhibition rate and IC50 of each compound on different cells were calculated by GraphPad Prism 8.0.1 software. Cell survival rate (%) = [(A (experimental group)-A (blank)]/[(A (DMSO)-A (blank)]× 100%.
8
Multimodal Cell Viability Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was analyzed using the CCK8 (TargetMol). The apoptosis and cell cycle were performed using the Annexin V-PE Apoptosis Detection Kit and APC BrdU Flow Kit (BD Pharmingen, San Diego, CA, USA). Data produced by the flow cytometer were analyzed using the FlowJo software.
9
Evaluating Cytotoxicity of Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCK8-assay was employed to evaluate the effect of the metabolites on the proliferation of cervical cancer cells. Cultured HeLa and GH354 cells suspended in a 100 μL culture medium with 10% FBS were inoculated in a 96-well plate (3000 cells/well) with 0, 5, 10, 20, and 30 μM C8 and C18. The cells with different concentrations of C8 and C18 were incubated for 24 hrs, 48 hrs, and 72 hrs. Before measuring the absorbance at 450 nm wavelength using a microplate reader, 10 μL CCK8 (TargetMOL, China) solution was added to each well and incubated for 1 hour.
10
Cell Viability Assay with CCK-8
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the cell viability assay, cells were seeded at a density of 1 × 105 cells per 100 μL in each well in 96-well microtiter plates (Corning, USA) and transfected with siRNA for 48 h. Cells were grown in each medium in triplicate. Then, 10 μL of reagent from the Cell Counting Kit-8 (CCK-8, TargetMol, USA) was added to each well, and the plates were incubated for 3 h at 37 °C. Cell viability was measured as the absorbance at 450 nm with a microplate reader (iMark™ Microplate Absorbance Reader, BioRad, USA). The mean optical density (OD) values from triplicate wells containing each culture medium were used as the cell viability indices.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!