Log In Sign up
The largest database of trusted experimental protocols

Explore locus sp scanner

Manufactured by GE Healthcare
Visit Supplier
Sourced in Canada

The EXplore Locus SP scanner is a small-animal PET/CT imaging system designed for preclinical research. It combines positron emission tomography (PET) and computed tomography (CT) modalities to provide high-resolution, functional and anatomical imaging of small animals. The scanner's core function is to enable non-invasive, longitudinal studies of biological processes in small animal models.

Automatically generated - may contain errors

Lab products found in correlation

Microview software, by GE Healthcare (1 mentions)

Spelling variants of this product

EXplore Locus SP (59 citations) EXplore Locus (31 citations) Locus SP (12 citations) EXplore Locus Scanner (4 citations) Scanners (2 citations) EXplore Locus SP Micro-CT scanner (2 citations) GE eXplore Locus SP Micro-CT Scanner (2 citations)

4 protocols using explore locus sp scanner

1

Comprehensive Microarchitectural Analysis of Metaphyseal Tibia

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The metaphyseal tibia was scanned with a micro-CT device (eXplore Locus SPScanner, GE Healthcare, Ontario, Canada) with 72 kVp, 90 μA, and 1,600 ms exposure time and 360° rotation, resulting in 0.0029 mm pixel size. Five hydroxyapatite blocks with defined mineral densities were scanned to transform gray scale values into bone mineral density (mg/cm3). 3D OsteoAnalyze, which was developed for our laboratory, was used to determine bone parameters according to American Society for Bone and Mineral Research (ASBMR) criteria (40 (link), 68 (link)–70 (link)). Total, trabecular and cortical BMD (g/cm2) as well as bone volume fraction (BV/TV) were assessed at the metaphysis. Therefore, two measurements next to the growth plate were performed (3 and 5 mm), and the area in between was cut out. The volume and density of cortical, trabecular and total bone were measured in this field. After turning 90°, the cross-section was used to measure the distal cortical bone area (Ct.Ar), endosteal area (E. Ar), and total area (T. Ar.) of the bone. Thereafter, the cortical bone was cut off, and trabecular structure was analyzed. Trabecular thickness (Tb.Th), number of trabecular nodes (N.Nd), trabecular number (Tb.N), mean trabecular junctions at one node (Tb.N/Nd), and trabecular separation (Tb.Sp) were measured (69 (link), 71 (link)).
Saul D., Geisberg L.K., Gehle T., Hoffmann D.B., Tezval M., Sehmisch S, & Komrakova M. (2019). Changes in Musculoskeletal System and Metabolism in Osteoporotic Rats Treated With Urocortin. Frontiers in Endocrinology, 10, 400.
+ Open protocol
+ Expand
2

In vivo Bone Structure Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine the three-dimensional bone structure in vivo, histomorphometric analyses were performed using a Micro-CT system (eXplore Locus SP scanner GE Healthcare) at 8 μm resolution. For details of micro-CT analysis, refer to the supplementary material method.
Lee S.J., Jeong J.Y., Oh C.J., Park S., Kim J.Y., Kim H.J., Doo Kim N., Choi Y.K., Do J.Y., Go Y., Ha C.M., Choi J.Y., Huh S., Ho Jeoung N., Lee K.U., Choi H.S., Wang Y., Park K.G., Harris R.A, & Lee I.K. (2015). Pyruvate Dehydrogenase Kinase 4 Promotes Vascular Calcification via SMAD1/5/8 Phosphorylation. Scientific Reports, 5, 16577.
+ Open protocol
+ Expand
3

High-Resolution Imaging of Bone Microstructure

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole left tibiae and femora (n=15/group) were scanned while submerged in Ca-PBS using an eXplore Locus SP scanner (GE Healthcare Pre-Clinical Imaging) at a voxel size of 18μm3 (80 kVp, 80 μA, 1600 ms, 0.508mm Al filter).(32 (link)) Calibration of the scanner using an air/water/Hydroxyapatite (HA) phantom was performed each day of scanning. Regions of interest were analyzed using a combination of GE MicroView software (reorientation, standard site selection, tissue mineral content (TMC) and tissue mineral density (TMD)) and custom-written MATLAB scripts (quantification of cortical geometry). Scans were reoriented to match the alignment of each bone in silico with its eventual alignment during mechanical testing. A 5-slice thick standard site was taken from each reoriented tibia at the point 23.5% of the distance from the tibia-fibula junction to the proximal end of the tibia, closely corresponding to the center of the 3mm mechanical testing span for all bones. Thus, the cortical site analyzed was reproducible from bone to bone and appropriate for calculating tissue level properties from 4-point testing data using classic beam theory. For the femur, a 5-slice standard site was located at the site 48% of its length as measured from the distal condyle. TMC and TMD were measured for both the femur and tibia using a fixed threshold of 2000 Hounsfield Units.
McNerny E.M., Gong B., Morris M.D, & Kohn D.H. (2015). Bone fracture toughness and strength correlate with collagen cross-link maturity in a dose-controlled lathyrism mouse model. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(3), 446-455.
+ Open protocol
+ Expand
4

Microcomputed Tomography Analysis of Patellar Enthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microcomputed tomography (μCT) analysis of the patella was performed prior to decalcification using the eXplore Locus SP scanner (GE Healthcare, London, ON, Canada). 2D images were acquired using an X-ray tube with a voltage of 80 kVP and a current of 80 μA, with a 0.508 mm Al filter. The exposure interval time of the scanner was 1600 msec/frame at 4 frames/view. In total, 900 views were obtained at 0.4 degrees angular increments. The data were reconstructed at a spatial resolution of 13.5 μm. All reconstructed data were calibrated with a cortical bone phantom (SB3; Gammex RMI, Milwaukee, WI, USA) with a hydroxyapatite equivalent of 1,100 mg/cc (White 1978), as well as water and air. Data were analyzed with MicroView ABA version 2.2 (GE Healthcare).
For patellar analysis, 5 mice per genotype were used. Quantification of mineral density was performed on 3D cubical volumes of interest (VOIs; 20 × 20 × 20 voxels) identified at the base area, where attachment of the quadriceps tendon occurred.
For measurement of mineral density distribution along the tendon-to-bone insertion, three parallel transverse line measurements were obtained and averaged for each region of interest (ROI). The lines were defined manually along the CFC of the QCT enthesis.
Marinovich R., Soenjaya Y., Wallace G.Q., Zuskov A., Dunkman A., Foster B.L., Ao M., Bartman K., Lam V., Rizkalla A., Beier F., Somerman M.J., Holdsworth D.W., Soslowsky L.J., Lagugné-Labarthet F, & Goldberg H.A. (2016). The Role of Bone Sialoprotein in the Tendon-Bone Insertion. Matrix biology : journal of the International Society for Matrix Biology, 52-54, 325-338.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!