Sabouraud dextrose broth sdb

Candida species were grown on SDA and incubated for 24 h at 37 °C. In order to prepare the inoculum, cells were then inoculated in SDB Sabouraud dextrose broth (SDB) (Merck, Darmstadt, Germany) and incubated for 18 h at 37 °C under agitation at 120 rpm. After incubation, the inoculum density was adjusted to 1 × 10⁵ cells/mL using a Neubauer chamber with RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA) [46 (link)].

Candida spp. were grown on SDA and incubated for 24 h at 37 °C. To prepare the inoculum, cells were then inoculated in SDB Sabouraud dextrose broth (SDB) (Merck, Darmstadt, Germany) and incubated for 18 h at 37 °C under agitation at 120 rpm. After incubation, the inoculum density was adjusted to 1 × 10⁵ cells/mL using a Neubauer chamber with RPMI-1640 (Sigma-Aldrich, St. Louis, MO, USA) [8 (link)].

In this study, two types of AuNPs were prepared by the biological method to compare the composition of their protein corona as well as of their capping agents. For this purpose, Sabouraud dextrose broth (SDB, Sigma-Aldrich, Prague, Czech Republic) was used to culture F. oxysporum (CCF 3732, Prague, Czech Republic) at 28 °C and 150 rpm for 1 week. The culture supernatant was obtained by centrifugation at 8000 rcf for 10 min [8 (link)], and AuNPs were prepared by adding HAuCl₄·3H₂O (Sigma-Aldrich, Prague, Czech Republic) at a final concentration of 1 mmol. The pH was adjusted to 7.5, and the mixture was divided into two flasks, one of which was incubated at 37 °C and 200 rpm for 24 h [24 (link)] (referred to here as “cold”) and the other at 80 °C for 5 min (referred to here as “hot”). Negative control vial containing sterile SDB plus 1 mmol HAuCl₄·3H₂O at the final concentration was subjected to the same procedures. The produced AuNPs were precipitated by centrifugation at 22,000 rcf for 20 min and washed three times with ddH₂O [9 (link),10 (link),25 (link)].