Ac yvad cmk sml0429

Sodium taurocholate (909688) was purchased from Bailingwei Technology Co., LTD. (Beijing, China) and emodin was obtained from Beijing Zhongke Quality Inspection (Beijing, China). Caerulein (C9026), LPS (L2880) and Ac-YVAD-CMK (SML0429) were purchased from Sigma-Aldrich (United States). Sodium pentobarbital (B5646) was purchased from Baoxin Biotechnology Co., LTD. (Chengdu, China). Anti-NLRP3 (ab263899), anti-GSDMD (ab219800) and anti-GSDMD-NT (ab215203) were obtained from Abcam (Milton, United Kingdom). Anti-ASC (DF-6304) and anti-Cleaved-Caspase 1 (p10, AF4022) were purchased from Affinity Biosciences (Jiangsu, China). Anti-GAPDH (10494-1-AP) was purchased from Proteintech Company (Chicago, United States).

Primary antibodies against caspase-1 (ab179515), caspase-11 (ab180673), GSDMD (ab209845), GSDME (ab215191) and Biotin Goat polyclonal to Chlamydia trachomatis (ab20387) were obtained from Abcam (Cambridge, UK), Antibody for Chlamydia HSP60 (sc57840) was bought from Santa Cruz Biotechnology (California, USA), Antibodies for caspase-8 (4790), caspase-8 p18 (8592), β-Tubulin (2128) and GAPDH (5174) were obtained from Cell Signaling Technology (Danvers, MA, USA), while secondary antibodies (SA00001-15, SA00001-1) used for immunoblotting and Fluorescein (FITC)-conjugated Affinipure Rabbit Anti-Goat IgG(H+L) (SA00003-4) used for immunofluorescence staining were bought from Proteintech (Chicago, USA). IL-1β (CSB-E08054m) and IL-18 (CSB-E04609m) ELISA kits were obtained from CUSABIO (Wuhan, China). Caspase-1 inhibitor Ac-YVAD-cmk (SML0429) was purchased from Sigma (California, USA), Necrosulfonamide (AG-CR1-3705-M005) was bought from Adipogen (Epalinges, Switzerland). Caspase-11 inhibitor Wedelolactone (T3384) was obtained from Target Mol (Boston, MA). All inhibitors were dissolved in dimethyl sulfoxide (DMSO).