Paraformaldehyde fixative

Manufactured by Solarbio

Sourced in China

4% paraformaldehyde fixative is a chemical solution used for the preservation and fixation of biological samples. It helps to maintain the structural integrity and morphology of cells, tissues, or organisms by cross-linking proteins. This solution is commonly used in various biological and medical applications, such as microscopy, histology, and immunohistochemistry.

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Lab products found in correlation

Nanozoomer scanner, by Hamamatsu Photonics (1 mentions) L dopa, by Merck Group (1 mentions)

4 protocols using paraformaldehyde fixative

Transwell Assay for Cell Migration and Invasion

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The migrative ability of the cells was determined using a transwell assay. For migration assays, add 2 × 10⁴ cells in 200 μL of serum-free medium to the upper chamber. Then, add 800 μL of medium containing 10% FBS to the bottom chamber. After 24 h of culture, the cells on the lower surface of the filter were fixed with 4% paraformaldehyde fixative (Solarbio), and then the invasive cells were counted with crystal violet staining.
The invasive ability of the cells was determined using a transwell assay. For invasion assays, add 2 × 10⁴ cells in 200 μL of serum-free medium to the upper chamber coated with Matrigel (San Jose, CA, USA). Then, add 800 μL of medium containing 10% FBS to the bottom chamber. After 24 h of culture, the cells on the lower surface of the filter were fixed with 4% paraformaldehyde fixative (Solarbio), and then the invasive cells were counted with crystal violet staining.

Li Z., Zhang H., Sun Y., Feng Z., Cui B., Han J., Li Y., Liu H, & Sun T. (2023). Live-cell imaging-based dynamic vascular formation assay for antivascular drug evaluation and screening. iScience, 26(5), 106721.

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Histological Analysis of Broiler Chicken Muscles

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Following the slaughter of broiler chickens, the NO and SM breast muscles selected through visual observation and touch, and the fascia and epidermis at the top of the left pectoralis major muscle were removed. Samples of the pectoral muscle tissue, aligned parallel to the muscle fibers (2 cm × 1 cm × 0.2 cm), were fixed in 4% paraformaldehyde fixative (Solarbio) for 48 h. Frozen samples were cross-sectioned and stained with hematoxylin and eosin (Khaliq, Beijing, China, 2023). Subsequently, samples were scanned using a Nano Zoomer scanner (Hamamatsu, Sydney, Australia). Images were acquired under the same conditions and magnification. Four fields of view were obtained per section, and three sections were acquired per sample. We used a microscope to observe the morphology of NO and SM muscle fibers.

Wu T., Liu P., Wu J., Jiang Y., Zhou N., Zhang Y., Xu Q, & Zhang Y. (2024). Broiler Spaghetti Meat Abnormalities: Muscle Characteristics and Metabolomic Profiles. Animals : an Open Access Journal from MDPI, 14(8), 1236.

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Melanocyte L-DOPA Staining Assay

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Melanocytes in the logarithmic growth phase were used to prepare sterile cell culture slides. The inoculated 24-well plates were cultured for 3 days and treated with 1 mL of 4% paraformaldehyde fixative (Solarbio, Beijing, Tongzhou, China) for 30 min at 4 °C. The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day, the incubation solution was renewed and the plates were further incubated at 37 °C for 12 h, with constant observation once every 30 min. The plates were washed with PBS once the staining was complete and observed under a microscope [37 (link)].

Chen Y., Hu S., Mu L., Zhao B., Wang M., Yang N., Bao G., Zhu C, & Wu X. (2019). Slc7a11 Modulated by POU2F1 is Involved in Pigmentation in Rabbit. International Journal of Molecular Sciences, 20(10), 2493.

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Mouse Maxilla Histological Analysis

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The unilateral mouse maxilla was dissected free of soft tissues, immersed in 4% paraformaldehyde fixative (Solarbio, Beijing, China), and fixed at room temperature for 48 h. After decalcification with 10% ethylene diamine tetraacetic acid (EDTA) (Solarbio, Beijing, China) at pH 7.4 for 14 days, the tissues were dehydrated, transparentized, embedded, sectioned, dewaxed, stained with hematoxylin–eosin (HE) and sealed with neutral gum. The samples were ready for microscopic examination and photography.

Song P., Hao Y., Lin D., Jin Y, & Lin J. (2024). Evaluation of the antibacterial effect of Epigallocatechin gallate on the major pathogens of canine periodontal disease and therapeutic effects on periodontal disease mice. Frontiers in Microbiology, 14, 1329772.

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