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2 protocols using tba 1 10 phenanthroline

1

Antioxidant and Radical Scavenging Assays

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Chemicals and reagents used such as thiobarbituric acid (TBA), 1,10‐phenanthroline, deoxyribose, gallic acid, Folin–Ciocalteau reagent were procured from Sigma‐Aldrich, Inc., (St. Louis, MO), trichloroacetic acid (TCA) was sourced from Sigma‐Aldrich, Chemie GmbH (Steinheim, Germany), hydrogen peroxide, methanol, sodium nitroprusside, Griess reagent, acetic acid, hydrochloric acid, sodium carbonate, aluminum chloride, potassium acetate, sodium dodecyl sulfate, iron (II) sulfate, potassium ferricyanide, and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, UK). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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2

Antioxidant and Reducing Potential Assays

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Chemicals and reagents used such as thiobarbituric acid (TBA), 1,10-phenanthroline, deoxyribose, gallic acid, and Folin-Ciocalteau's reagent were procured from Sigma-Aldrich, Inc. (St Louis, MO), trichloroacetic acid (TCA) was sourced from Sigma-Aldrich, Chemie GmbH (Steinheim, Germany), dinitrophenylhydrazine (DNPH) was sourced from ACROS Organics (New Jersey, USA), and hydrogen peroxide, methanol, acetic acid, thiourea, CuSO4·5H2O, H2SO4, HCl, sodium carbonate, AlCl3, potassium acetate, Tris-HCl buffer, sodium dodecyl sulphate, FeSO4, potassium ferricyanide, and ferric chloride were sourced from BDH Chemicals Ltd. (Poole, England), while the water was glass distilled.
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