Survivin antibody

Manufactured by Cell Signaling Technology

Sourced in United States

Survivin antibody is a laboratory reagent used for the detection and study of the survivin protein. Survivin is a member of the inhibitor of apoptosis (IAP) protein family and plays a role in regulating cell division and inhibiting apoptosis. The survivin antibody is a tool for researchers to study the expression and function of survivin in various biological systems.

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Close spelling variants of this product

Survivin (307 citations) Anti-survivin antibody (11 citations) Antibody against survivin (2 citations)

5 protocols using survivin antibody

Comprehensive Antibody Panel for Cellular Signaling

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S6K1 antibody, phospho-S6K1 (Thr389) antibody, phospho-S6K1 (Thr421/Ser424) antibody, 4E-BP1 antibody, phospho-4E-BP1(Thr37/46) antibody, phospho-4E-BP1 (Ser65) antibody, phospho-4E-BP1 (Thr70) antibody, eIF4E antibody, phospho-eIF4E (Ser209) antibody, p44/42 MAP kinase antibody, phospho-p44/42 MAP kinase (Thr202/Tyr204) antibody, phospho-Akt (Ser473) antibody, Akt antibody, phospho-PKA substrate (RRXS*/T*) (100G7E) antibody, phospho-(Ser/Thr) PKA substrate antibody, c-Myc antibody, cyclin B1 antibody, survivin antibody, β-actin antibody, and goat-rabbit IgG conjugated with HRP were purchased from Cell Signaling Technology. Cyclin D3 rabbit polyclonal antibody was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). CD3 antibody (FITC-conjugated), CD13 antibody (PE conjugated), CD19 antibody (perCP conjugated), CD33 antibody (PE conjugated), CD34 antibody (PE-conjugated), CD41a antibody (PE-conjugated), CD42a antibody (FITC conjugated), CD42b antibody (FITC conjugated), CD71 antibody (FITC conjugated) and CD235a (FITC conjugated) were purchased from Becton Dickinson (BD).

Li C.L., Yang J.G., Lin D., Zhao Y.S., Liu S., Xing S.N., Zhao S., Chen C.Q., Jiang Z.M., Pu F.F., Cao J.P, & Ma D.C. (2014). Phosphorylation of Ribosomal Protein S6 Kinase 1 at Thr421/Ser424 and Dephosphorylation at Thr389 Regulates SP600125-Induced Polyploidization of Megakaryocytic Cell Lines. PLoS ONE, 9(12), e114389.

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Comprehensive Western Blotting Protocol

Cited 1 time

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Western blotting was performed as described previously [13 (link)]. The following antibodies were used: CCN1 antibody (Abcam), β-actin antibody (Santa Cruz Biotechnology), Bcl-xL antibody (Cell Signaling Technology), c-Myc antibody (Cell Signaling Technology), Bax antibody (Santa Cruz Biotechnology), MEK antibody (Cell Signaling Technology), phospho-MEK antibody (Ser217/221, Cell Signaling Technology), ERK antibody (Cell Signaling Technology), phospho-ERK antibody (Thr202/Tyr204, Cell Signaling Technology), β-catenin antibody (Cell Signaling Technology), phospho-β-catenin antibody (Ser33/Ser37/Thr41, Cell Signaling Technology), and Survivin antibody (Cell Signaling Technology).
Gel electrophoresis and transfer as well as the chemiluminescence detection were conducted using the Bio-Rad Laboratories system.
Nuclear protein was extracted using a nuclear protein extraction kit (Beyotime, China), and an anti-Histone H1 antibody (Santa Cruz Biotechnology) was used as the loading control.

Niu C.C., Zhao C., Yang Z., Zhang X.L., Pan J., Zhao C, & Si W.K. (2014). Inhibiting CCN1 blocks AML cell growth by disrupting the MEK/ERK pathway. Cancer Cell International, 14, 74.

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MTT Assay of LLL12 in DMSO

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LLL12 was provided by the Ohio State University College of Pharmacy and dissolved in DMSO. For the MTT assay (Research Products International, Mount Prospect, IL), the following antibodies were used: phospho-Stat3 (Y705) antibody, Stat3 antibody, cleaved caspase-3 (Asp175) antibody, cyclin D1 antibody, survivin antibody, and biotinylated glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (14C10) rabbit antibody (Cell Signaling Technology, Beverly, MA).

Zuo M., Li C., Lin J, & Javle M. (2015). LLL12, a novel small inhibitor targeting STAT3 for hepatocellular carcinoma therapy. Oncotarget, 6(13), 10940-10949.

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Immunohistochemistry of Rab18, p-Rb, and Survivin

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Paraffin tissue sections were made, and immunostaining was performed using Elivision kit from MaiXin (Fuzhou, China). After antigen retrieval in citrate buffer (pH 6.0) for 2 minutes in an autoclave, 0.3% hydrogen peroxide was added for 10 minutes. Sections were incubated with goat serum (ready-to-use; MaiXin) to reduce nonspecific binding. Then, sections were incubated with Rab18 antibody (1:300 dilution; Proteintech, Rosemont, IL, USA), p-Rb antibody (1:200 dilution; Cell Signaling Technology, Danvers, MA, USA), and survivin antibody (1:400 dilution; Cell Signaling Technology) at 4°C overnight. After incubation, horse radish peroxidase (HRP)-conjugated polymers were applied to sections. DAB-Plus kit (MaiXin) was used for staining.

Wu B., Qi R., Liu X., Qian L, & Wu Z. (2018). Rab18 overexpression promotes proliferation and chemoresistance through regulation of mitochondrial function in human gastric cancer. OncoTargets and therapy, 11, 7805-7820.

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Profiling Cancer Cell Lines

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Breast (SKBR3, MDA-MB-231), kidney (786-O), colorectal cancer (SW480), lung (A549), and pancreatic (Panc1, L3.6pL, MiaPaCa2) cancer cell lines were purchased from American Type Culture Collection (Manassas, VA). Cells were maintained 37°C in the presence of 5% CO₂ in Dulbecco's Modified Eagle's Medium/Ham's F-12 medium with 10% fetal bovine serum with antibiotic or RPMI-1640 Medium with 10% fetal bovine serum and antibiotic. b-Actin antibody, Dulbecco's Modified Eagle's Medium, and RPMI-1640 Medium, and 36% formaldehyde were purchased from Sigma-Aldrich (St. Louis, MO). Hematoxylin was purchased from Vector Laboratories (Burlingame, CA). Sp1 antibody from Millipore (Temecula, CA); Sp3, Sp4, EGFR, bcl2 antibodies from Santa Cruz Biotech (Santa Cruz, CA); survivin antibody from Cell Signaling Technologies (Danvers, MA); VEGF antibody from GeneTex (Irvine, CA). Apoptotic, Necrotic, and Healthy Cells Quantification Kit was purchased from Biotium (Hayward, CA). Cells were visualized as described previously [21 (link)].

Hedrick E., Cheng Y., Jin U.H., Kim K, & Safe S. (2016). Specificity protein (Sp) transcription factors Sp1, Sp3 and Sp4 are non-oncogene addiction genes in cancer cells. Oncotarget, 7(16), 22245-22256.

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