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Alphascreen surefire erk assay

Manufactured by PerkinElmer
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The AlphaScreen SureFire ERK assay is a laboratory equipment product from PerkinElmer. It is a homogeneous, no-wash assay that measures the activation of extracellular signal-regulated kinase (ERK) in cellular samples.

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AlphaScreen SureFire p-ERK assay kit AlphaScreen SureFire assay AlphaScreen assay

2 protocols using alphascreen surefire erk assay

1

Quantification of Activated ERK Levels

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Detection of activated ERK in cell lysates was enabled by the AlphaScreen SureFire ERK assay (Perkin Elmer) according to the manufacturers protocol. Cells were plated in 96-well plates for 24 hours prior to the assay. Cells were treated with either vehicle or indicated concentrations of V-9302 or CB-839 for 48 hours.
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
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2

Quantification of Activated ERK Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Detection of activated ERK in cell lysates was enabled by the AlphaScreen SureFire ERK assay (Perkin Elmer) according to the manufacturers protocol. Cells were plated in 96-well plates for 24 hours prior to the assay. Cells were treated with either vehicle or indicated concentrations of V-9302 or CB-839 for 48 hours.
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
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