The concentration of timolol maleate in the liquid ink was determined using HPLC-UV, equipped with a Hewlett Packard 1260 Series HPLC system (Agilent Technologies, Cheadle, UK). The stationary phase was an Eclipse Plus C18 Column 5 μm, 150 × 4.6 μm (Agilent Technologies, Cheadle, UK), and the mobile phase was a combination of 0.01 M ammonium acetate (pH 5.0) and methanol at a ratio of 60:40 v/v. The aqueous solution was prepared by adding ammonium acetate (0.7708 g) to Type 1 water (1.0 L) and adjusting the pH with concentrated hydrochloric acid. The flow rate was set to 1.0 mL/min, with a column temperature of 40 °C, an injection volume of 50 μL and a UV-wavelength of 295 nm. The elution time of timolol maleate was approximately 3.4 min. A calibration curve for timolol maleate in solution was prepared between 0.4 and 400 μg/mL (R2 = 0.99999). The solutions were stored in sealed 1.5 mL amber glass vials (Fisher Scientific, Loughborough, UK), and 0.1 mL 5 × 31 mm glass inserts (Macherey-Nagel GmbH & Co KG, Düren, Germany) were used for samples of <1.0 mL.
Amber glass vials
Manufactured by Thermo Fisher Scientific
Sourced in United States
Amber glass vials are a type of laboratory container designed to store and protect light-sensitive materials. They are made from amber-colored glass, which helps to block ultraviolet and visible light, preventing the degradation of the contents.
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Lab products found in correlation
5 protocols using amber glass vials
1
Quantification of Timolol Maleate in Liquid Ink
2
Fluorescent Polymer Nanoparticle Synthesis
3
Essential Oil Extraction and Characterization
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The essential oils (EOs) tested from Satureja montana L. (SM) and Origanum virens Hoffmanns and Link (OV) were provided by The Agricultural Research Centre of Albaladejito (Cuenca, Spain). Extraction was previously described by García-Díaz et al. (2019) [25 (link)]. Briefly, each plant species was extracted by hydrodistillation of the dried aerial parts of aromatic plants, following the methodology proposed by the European Pharmacopoeia in a Clevenger-type apparatus for 2 h. The chromatograms are shown in Appendix B .
These compounds were filtered with sterile 0.22 μm pore size filters (Fisher Scientific, Madrid, Spain) and stored at −20 °C in amber glass vials (Thermo Scientific, Madrid, Spain), until required.
These compounds were filtered with sterile 0.22 μm pore size filters (Fisher Scientific, Madrid, Spain) and stored at −20 °C in amber glass vials (Thermo Scientific, Madrid, Spain), until required.
4
Moringa Phenolic Compounds Extraction
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Extraction and analysis of phenolic compounds were carried according to the protocol described by [17] (link). To the 0.1 g of each fine moringa powder sample, 3 mL of aqueous MeOH solution (80%) was added and vortexed for 1 min, which was then sonicated for 1 h at 37 • C. After that, the mixture was centrifuged at 4 • C for 15 min at 10,000 rpm. The clear supernatants were passed through a 0.45 µm PTFE syringe filter (Millipore, Bedford, MA, USA) into amber glass vials (Thermo Fisher Scientific, Waltham, MA, USA). The HPLC machine, mobile phase, gradient program, identification, and quantification of the phenolic compounds were similar to the protocol described in [22] (link).
5
Moringa Phenolic Compounds Extraction
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Extraction and analysis of phenolic compounds were carried according to the protocol described by [17] (link). To the 0.1 g of each fine moringa powder sample, 3 mL of aqueous MeOH solution (80%) was added and vortexed for 1 min, which was then sonicated for 1 h at 37 • C. After that, the mixture was centrifuged at 4 • C for 15 min at 10,000 rpm. The clear supernatants were passed through a 0.45 µm PTFE syringe filter (Millipore, Bedford, MA, USA) into amber glass vials (Thermo Fisher Scientific, Waltham, MA, USA). The HPLC machine, mobile phase, gradient program, identification, and quantification of the phenolic compounds were similar to the protocol described in [22] (link).
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