Trifluroacetic acid

Manufactured by Agilent Technologies

Trifluoroacetic acid (TFA) is a colorless, corrosive liquid used in various laboratory applications. It is a strong organic acid with a boiling point of 72.4°C. TFA is commonly utilized as a reagent in organic synthesis, peptide synthesis, and protein purification processes.

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Lab products found in correlation

Gibberellic acid, by Merck Group (2 mentions) Jasmonic acid, by Merck Group (2 mentions) Tryptophan 15n2, by Merck Group (2 mentions) Trans zeatine, by Merck Group (2 mentions) 15n anthranilic acid, by Merck Group (2 mentions) Acetonitrile, by Agilent Technologies (1 mentions) Methanol, by Avantor (1 mentions) Formic acid and ammonium acetate, by Merck Group (1 mentions) Sarcosine d3, by Merck Group (1 mentions) Thymine d4, by Merck Group (1 mentions) Testosterone d3, by Merck Group (1 mentions) Hplc grade acetonitrile and water, by Avantor (1 mentions) N acetyl aspartic acid d3, by Merck Group (1 mentions) Glutamic acid d5, by Merck Group (1 mentions)

2 protocols using trifluroacetic acid

Metabolomic Analysis of Plant Hormones

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High-performance liquid chromatography (HPLC)-grade acetonitrile, methanol, and water were procured from Burdick & Jackson (Morristown, NJ). Mass spectrometry-grade formic acid was purchased from Sigma-Aldrich (St Louis, MO). Calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water was purchased from Agilent Technologies (Santa Clara, CA). Metabolites and internal standards, including N-acetyl Aspartic acid-²H3, Tryptophan-¹⁵N2, Sarcosine-²H3, Glutamic acid-²H5, Thymine-²H4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, Anthranilic acid 15N, and Testosterone-²H3, were purchased from Sigma-Aldrich (St. Louis, MO).

Colleluori G., Aguirre L., Phadnis U., Fowler K., Armamento-Villareal R., Sun Z., Brunetti L., Park J.H., Kaipparettu B.A., Putluri N., Auetumrongsawat V., Yarasheski K., Qualls C, & Villareal D.T. (2019). Aerobic plus Resistance Exercise in Obese Older Adults Improves Muscle Protein Synthesis and Preserves Myocellular Quality Despite Calorie Restriction. Cell metabolism.

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Comprehensive Metabolomics Analytical Protocol

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Sources for reagents were: HPLC-grade acetonitrile and water (Burdick & Jackson); mass spectrometry-grade formic acid and ammonium acetate (Sigma-Aldrich); calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water (Agilent Technologies); metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, Tryptophan-15N2, Sarcosine-d3, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, 15N Anthranilic acid, and Testosterone-d3 (Sigma-Aldrich).

Amobi-McCloud A., Muthuswamy R., Battaglia S., Yu H., Liu T., Wang J., Putluri V., Singh P.K., Qian F., Huang R.Y., Putluri N., Tsuji T., Lugade A.A., Liu S, & Odunsi K. (2021). IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation. Frontiers in Immunology, 12, 678999.

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