Anti cd68 antibody clone pg m1

Manufactured by Agilent Technologies

Sourced in United States

The Anti-CD68 antibody (clone PG-M1) is a laboratory reagent used for the detection and identification of the CD68 antigen, which is commonly expressed on macrophages and monocytes. This antibody can be used in various immunohistochemical and flow cytometry applications to study the distribution and expression of CD68-positive cells in biological samples.

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Lab products found in correlation

Glycerol gelatin, by Merck Group (2 mentions) Dako real detection system, by Agilent Technologies (2 mentions) Axio imager z1 microscope, by Zeiss (2 mentions) Anti cd20 clone l26, by Agilent Technologies (1 mentions) Anti cd138 clone mi15, by Agilent Technologies (1 mentions) Anti mum1 clone mum1p, by Agilent Technologies (1 mentions) Mib1 clone mib1, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Anti-CD68 (66 citations) PG-M1 (51 citations) CD68 (clone PG-M1, (36 citations) Anti-CD68 (clone PG-M1, (14 citations) Clone PG-M1 (13 citations) Anti-CD68 antibody (13 citations) Anti-CD68 (PG-M1, (4 citations) CD68 antibody (4 citations) Anti-TM (1 citations)

5 protocols using anti cd68 antibody clone pg m1

Immunohistochemical Staining of M-CSFR, M-CSF, and IL-34

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Single and double immunohistochemical staining was performed as described
previously.²⁰ (link) In brief, deparaffinized
sections were microwave treated in 1 mM EDTA (pH 8.0). Then, sections were reacted with
anti-M-CSFR antibody (clone SP211, Abcam, Cambridge, UK), anti-M-CSF antibody (clone
EP1179Y, Abcam), or isotype-matched control antibody (DAKO, Glostrup, Denmark). For IHC of
macrophages, anti-CD68 antibody (clone PG-M1, DAKO) was used. IHC for IL-34 was performed
as described previously.²¹ (link) Horseradish
peroxidase (HRP)-labeled anti-mouse or rabbit immunoglobulin antibody (Nichirei, Tokyo,
Japan) was used as the secondary antibody. Diaminobenzidine (DAB, brown color) and
HistoGreen (green color) substrate (#AYS-E109, Cosmo Bio, Tokyo, Japan) were used for
visualization of positive signals.

Komohara Y., Noyori O., Saito Y., Takeya H., Baghdadi M., Kitagawa F., Hama N., Ishikawa K., Okuno Y., Nosaka K., Seino K.I., Matsuoka M, & Suzu S. (2018). Potential anti-lymphoma effect of M-CSFR inhibitor in adult T-cell leukemia/lymphoma. Journal of Clinical and Experimental Hematopathology : JCEH, 58(4), 152-160.

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Comprehensive Bone Marrow Biopsy Analysis in MM

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In all cases, bone marrow biopsy evaluation was performed as a part of the staging workup for MM at iliac spine. In selected patients (cases 16, 17, and 18), typical HU < 0 LBLs underwent to needle biopsy. In detail, 3-μm-thick slides were obtained from formalin-fixed, decalcified paraffin-embedded marrow samples. Following the routine protocol for bone marrow examination, the slices were stained with H&E, PAS, and Giemsa. Marrow fibrosis was assessed by reticulin stain. The immunohistochemical characterization of plasma cells was performed using the following antibodies: anti-CD138 (clone MI15 Dako, Glostrup, Denmark), anti-MUM1 (clone MUM1p, Dako, Glostrup, Denmark), anti-CD56 (clone 504 Leica, Milan, Italy), anti-cyclin D1 (clone EP12, Dako, Glostrup, Denmark), anti-CD20 (clone L26, Dako, Glostrup, Denmark), anti-CD79a (clone 11E3, Leica, Milan, Italy), anti-CD3 (clone LN10, Novocastra, Milan, Italy), and Mib1 (clone Mib1 Dako, Glostrup, Denmark). An anti-CD68 antibody (clone PGM1, Dako, Glostrup, Denmark) was used to assess the presence and distribution of peri-trabecular osteoclasts.

Zambello R., Crimì F., Lico A., Barilà G., Branca A., Guolo A., Varin C., Vezzaro R., Checuz L., Scapin V., Berno T., Pizzi M., Ponzoni A., De Biasi E., Vio S., Semenzato G., Zucchetta P, & Lacognata C. (2018). Whole-body low-dose CT recognizes two distinct patterns of lytic lesions in multiple myeloma patients with different disease metabolism at PET/MRI. Annals of Hematology, 98(3), 679-689.

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Quantifying Muscle Inflammation via IHC

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In order to evaluate the presence of inflammatory cells in diaphragm and gastrocnemius muscles, immunohistochemical analyses were conducted in each muscle of all study groups following previously published methodologies and specific antibodies (Barreiro et al., 2011 (link); Barreiro et al., 2010 (link)). On three-micrometer muscle paraffin-embedded sections, leukocytes (anti-CD45 antibody, clone 2B11 & PD7/26; Dako Cytomation Inc., Carpinteria, CA, USA) and macrophages (anti-CD68 antibody, clone PG-M1, Dako Cytomation Inc.) were identified following immunohistochemical procedures (Barreiro et al., 2011 (link); Barreiro et al., 2010 (link)). Results corresponding to inflammatory cell counts were expressed as follows: the ratio of either leukocyte or macrophage numbers to total muscle section area and the ratio of both cell types to total muscle section area in both diaphragm and gastrocnemius muscles (Barreiro et al., 2011 (link)).

Salazar-Degracia A., Busquets S., Argilés J.M., López-Soriano F.J, & Barreiro E. (2017). Formoterol attenuates increased oxidative stress and myosin protein loss in respiratory and limb muscles of cancer cachectic rats. PeerJ, 5, e4109.

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Immunohistochemical Analysis of CD68+ Cells

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For immunohistochemistry, 5 µm cryo-sections of human left ventricle were fixed in formalin for 1 hour at room temperature and subjected to a heat-induced epitope retrieval step prior to incubation with anti-CD68 antibody (clone PGM-1, Agilent Technologies, Santa Clara, CA, USA). The detection was performed by the LSAB method applying the Dako REAL™ Detection System (Agilent Technologies, Santa Clara, CA, USA). Nuclei were counterstained with hematoxylin and mounted on slides with glycerol gelatin (both Merck KGaA, Darmstadt, Germany). Negative controls were performed by omitting the primary antibody. Images were acquired using an AxioImager Z1 microscope (Carl Zeiss MicroImaging, Inc.). Positive cells were quantified in 5 high power fields (hpf) (field of vision in x400 original magnification). All evaluations were performed in a blinded manner.

Barcena de Arellano M.L., Pozdniakova S., Kühl A.A., Baczko I., Ladilov Y, & Regitz-Zagrosek V. (2019). Sex differences in the aging human heart: decreased sirtuins, pro-inflammatory shift and reduced anti-oxidative defense. Aging (Albany NY), 11(7), 1918-1933.

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Immunohistochemical Analysis of CD68 in Human LV

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For immunohistochemistry, 5 μm cryo-sections of human LV were fixed in formalin for 1 hour at room temperature and subjected to a heat-induced epitope retrieval step prior to incubation with anti-CD68 antibody (clone PGM-1, Agilent Technologies, Santa Clara, CA, USA). The detection was performed by the LSAB method applying the Dako REAL™ Detection System (Agilent Technologies, Santa Clara, CA, USA). Nuclei were counterstained with hematoxylin and mounted on slides with glycerol gelatin (both Merck KGaA, Darmstadt, Germany). Negative controls were performed by omitting the primary antibody. Images were acquired using an AxioImager Z1 microscope (Carl Zeiss MicroImaging, Inc.). Positive cells were quantified in 10 high power fields (hpf) (field of vision in x400 original magnification). All evaluations were performed in a blinded manner.

Barcena M.L., Pozdniakova S., Haritonow N., Breiter P., Kühl A.A., Milting H., Baczko I., Ladilov Y, & Regitz-Zagrosek V. (2020). Dilated cardiomyopathy impairs mitochondrial biogenesis and promotes inflammation in an age- and sex-dependent manner. Aging (Albany NY), 12(23), 24117-24133.

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