miR-520b mimics(miR-520b), inhibitor(inh), miR-NC, inhibitor-NC(inhNC) were synthesized and purified by RiboBio (Guangzhou, China). The concentration of miR-520b, inh or inhNC was 100nM. Small interfering RNA targeting MLK3 (Si-MLK3) and negative control (Si-NC) were obtained from OriGene Technologies (Rockville, MD, USA). The concentration of Si-NC and Si-MLK3 was 100nM. Lipofecatmine 2000 (Invitrogen, USA) was used as transfection reagent as manufacture suggested. The experiments were done 48h after transfection unless clarified.
Mir 520b
Manufactured by RiboBio
Sourced in China
The MiR-520b is a laboratory instrument used for the detection and analysis of miR-520b, a specific microRNA molecule. This device provides researchers with the ability to quantify the expression levels of miR-520b in biological samples. The core function of the MiR-520b is to facilitate the measurement and study of this particular microRNA.
Automatically generated - may contain errors
Lab products found in correlation
Lipofecatmine 2000, by Thermo Fisher Scientific (1 mentions)
Inhibitor nc, by RiboBio (1 mentions)
Si nc, by Thermo Fisher Scientific (1 mentions)
Si nc, by OriGene (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Si hdac4, by RiboBio (1 mentions)
Anti mir 520b, by RiboBio (1 mentions)
3 protocols using mir 520b
1
Regulation of MLK3 by miR-520b
2
miR-520b Regulation of HDAC4 Expression
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The cells were grown on 6‐well, 24‐well, or 96‐well plates for 24 hours. Lipofectamine 2000 (Invitrogen) was used as a reagent to perform transient transfection of miRNAs or small interfering RNAs (siRNAs). MiR‐520b, control (Ctrl), MiR‐520b inhibitor, and si‐HDAC4 were purchased from RiboBio (Guangzhou, China).
3
Evaluating miR-520b Inhibition Effects
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A549 cells (3,000 cells/well) were grown on 96-well plates for 24 h. miR-520b, anti-miR-520b, or anti-miR-520b and si-FGF16 (RiboBio, Guangzhou, China) was transfected into the cells with at least three replicates through lipofectamine 2000. At the indicated time point (0, 24, 48, or 72 h), 10 μL MTT (5 mg/mL) was supplied into the wells. The absorbance values at OD490nm were determined by the absorbance reader.
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