Rabbit anti β galactosidase

Manufactured by Merck Group

Sourced in United States

Rabbit anti-β-Galactosidase is a primary antibody raised in rabbits against the β-Galactosidase protein. It is commonly used in various immunoassays and molecular biology techniques to detect and quantify the presence of β-Galactosidase in biological samples.

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Lab products found in correlation

Alexa fluor secondary antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Lsm 510, by Zeiss (2 mentions) Desmin, by Santa Cruz Biotechnology (1 mentions) Dystrophin, by Merck Group (1 mentions) Laminin, by Merck Group (1 mentions) Fv1000 confocal microscopy, by Olympus (1 mentions) M o m kit, by Vector Laboratories (1 mentions) Volocity, by PerkinElmer (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions) Mouse anti gfp, by Thermo Fisher Scientific (1 mentions) Mouse anti β galactosidase, by Merck Group (1 mentions) Alexa fluor 647 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)

5 protocols using rabbit anti β galactosidase

Immunofluorescence Analysis of Skeletal Muscle

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Frozen muscle sections were fixed in 4% paraformaldehyde and permeabilized in 0.5% Triton X-100 for 10 min. Sections were incubated with mouse IgG-blocking solution from the M.O.M kit (Vector Lab, Burlingame, CA, USA) according to the manufacturer's protocol. Primary and secondary antibodies were as following: Desmin (1 : 200, Santa Cruz, Dallas, TX, USA), dystrophin (1 : 200, Sigma-Aldrich), Laminin (1 : 500, Sigma-Aldrich), MF20 (1 : 10, DSHB), Pax7 (1 : 100, DSHB), eMHC (1 : 200, DSHB), BA-F18 (1 : 2, DSHB), BAD5 (1 : 2, DSHB), and Rabbit anti β-galactosidase (1 : 500, Sigma-Aldrich). FITC-conjugated F4/80 and CD11b (eBiosciences, San Diego, CA, USA) were used for staining macrophage markers in cardiotoxin injured TA muscles. All secondary antibodies were obtained from Invitrogen (Carlsbad, CA, USA) and used at 1 : 500 dilutions. Pictures were taken with a Nikon TE2000 epifluorescent microscope with deconvolution (Volocity; Perkin-Elmer, Waltham, MA, USA) or an Olympus FV1000 confocal microscopy (FV1000, Olympus, Center Valley, PA, USA).

Nie M., Liu J., Yang Q., Seok H.Y., Hu X., Deng Z.L, & Wang D.Z. (2016). MicroRNA-155 facilitates skeletal muscle regeneration by balancing pro- and anti-inflammatory macrophages. Cell Death & Disease, 7(6), e2261-.

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Immunohistochemistry Assay for Fly Markers

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Immunohistochemistry and image acquisition were carried out as previously described (Sun and Deng, 2005 (link)). The following antibodies were used: mouse anti-Br-Core (25E9) 1:30, mouse anti-Br-Z1 (3C11) 1:30, mouse anti-Hnt (1G9) 1:15, anti-CycB (F2F4) 1:50, anti-Cut (2B10) 1:15 (Development Studies Hybridoma Bank, USA), rabbit anti-β-Galactosidase 1:5000 (Sigma, USA), rabbit anti-PH3 1:200 (Upstate Biotechnology, NY, USA). Images were acquired with a Zeiss LSM 510 confocal microscope and processed in Photoshop and Image J. Signal intensity was measured by the Interactive 3D Surface Plot Plugin of Image J.

Jia D., Tamori Y., Pyrowolakis G, & Deng W.M. (2014). Regulation of broad by the Notch pathway affects timing of follicle cell development. Developmental biology, 392(1), 52-61.

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Immunohistochemistry in Drosophila Ovaries

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Immunohistochemistry was carried out as previously described in Drosophila ovaries (Jia et al., 2014 (link), 2015 (link)). The following antibodies were used: mouse anti-Br-Core (25E9) 1:30, anti-Cut (2B10) 1:15 (Development Studies Hybridoma Bank, USA), rabbit anti-β-Galactosidase 1:5000 (Sigma, USA). Corresponding Alexa Fluor secondary antibodies (1:400; Invitrogen, USA) were selected according to primary antibodies.

Jia D., Bryant J., Jevitt A., Calvin G, & Deng W.M. (2016). The Ecdysone and Notch Pathways Synergistically Regulate Cut at the Dorsal-Ventral Boundary in Drosophila Wing Discs. Journal of genetics and genomics = Yi chuan xue bao, 43(4), 179-186.

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Immunostaining Fly Imaginal Discs

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Immunostainings of fly imaginal discs was performed by standard protocol. The following antibodies were used in this study: rabbit anti-β-galactosidase (Cappel), mouse anti-β-galactosidase (Sigma-Aldrich, #G4644), guinea pig anti-Dll [9 (link)], rat anti-Sp1, guinea pig anti-Hth [54 (link)], mouse-anti-GFP (ThermoFisher Scientific, #A11121), rat anti-C15 [15 (link)], rat anti-Al [6 (link)], rat anti-BarH1 [55 (link)], rabbit anti-BarH1 [56 (link)], mouse anti-Dac [57 (link)]. AlexaFluor488-, AlexaFluor555-, and AlexaFluor647-conjugated secondary antibodies from ThermoFisher Scientific or Jackson ImmunoResearch Laboratories were used at 1⫶500 dilution.
Adult legs were dissected, mounted, and analyzed by light microscopy. All adults of the relevant genotype that eclosed within an 8-hour period were scored. Roman numerals in the figure legends indicate the tarsal segments present in each phenotypic class (with the distal most segment perturbed). For example, a truncation designated as I-III means that tarsal segments I, II and III were present, with segment III partially defective (e.g. Fig 2P). n refers to the number of individual legs scored. The number of legs examined for each genotype is reported in the figures and figure legends.

Newcomb S., Voutev R., Jory A., Delker R.K., Slattery M, & Mann R.S. (2018). cis-regulatory architecture of a short-range EGFR organizing center in the Drosophila melanogaster leg. PLoS Genetics, 14(8), e1007568.

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Immunohistochemistry Staining and Imaging

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Immunohistochemistry and image acquisition were performed as previously described (Jia et al., 2015 (link); Sun and Deng, 2005 (link)). The following antibodies were used: mouse anti-Br-Core (25E9) 1:30, mouse anti-Arm (N2 7A1) 1:40 (Development Studies Hybridoma Bank, USA), rabbit anti-β-Galactosidase 1:5000 (Sigma, USA). Corresponding Alexa Fluor secondary antibodies (1:400; Invitrogen) were selected according to primary antibodies. Images were acquired with Zeiss confocal microscopes (LSM 510 at Florida State University, LSM 700 at Georgia Southern University, LSM 800 at Tulane University) and processed in Photoshop and Image J. Signal intensity was measured by the Interactive 3D Surface Plot Plugin of Image J. To generate line profiles, images were opened in Image J, a 12 μm line was drawn perpendicular to the stretched cell layer using the straight line tool, and the Intensity Profile tool was used to plot the profile.

Jia D., Jevitt A., Huang Y.C., Ramos B, & Deng W.M. (2022). Developmental regulation of epithelial cell cuboidal-to-squamous transition in Drosophila follicle cells. Developmental biology, 491, 113-125.

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