P 4e bp t37 46

P-TSC2 S939 (LS-C358381) was from Biozol (Sontheim, Germany). Antibodies specific for TSC2/tuberin (#4308), P-AKT S473 (#4060), AKT (#4691), P-S6K T389 (#9234), P-S S235/6 (#2211), S6 (#2217), P-ERK T202/Y204 (#4370), ERK (#4696), P-4E-BP T37/46 (#2855), as well as MG132 and cyclohexamide were from Cell Signaling (Danvers, MA, USA). Involucrin (SY8; ab20202) was from Abcam (Cambridge, UK), Actin antibody (A1978) was from Sigma-Aldrich (St. Louis, MO, USA) and LAMP-2 antibody (sc-18822) from SantaCruz Biotechnology (Dallas, TX, USA). LY294002, U0126 were from were from Calbiochem (San Diego, CA, USA).

Cells were lysed using modified RIPA buffer (50 mM Tris–HCl, 150 mM NaCl, 1% NP-40, pH 8.0, 1 × protease inhibitor cocktail) and lysates were subjected to western blotting with the indicated primary antibodies. Antibodies to HK1, HK2, Di-methyl-H3-Lys4, Acetyl-H3-Lys9, LSD1, DNMT1, CHD1, CHD3, CHD4, MTA1, AKT, pAKTS473, mTOR, p-mTOR S2448, pS6K T398, p4E-BP T37/46, and HDAC1, 2, 3, 6 were purchased from cell signaling technologies (Beverly, MA, USA). Antibodies to IDH1, IDH2, and SDHA were from GeneTex, Inc. (Irvine, CA, USA). Anti-PHF14 antibody was from Proteintech Group, Inc. (Rosemont, IL, USA). Actin and GAPDH antibodies were obtained from Millipore (Billerica, MA, USA). Proteins bound by these antibodies were detected using a chemiluminescent detection kit (Pierce; Thermo Fisher Scientific, Inc., Waltham, MA, USA).