The wild-type and mutant constructs of EIF3H plasmids were purchased from the Hanbio Biotechnology Co., Ltd. (Shanghai, China). The HA-K48, HA-K63, and HA-Ub plasmids were acquired from Addgene. The small interfering RNAs targeting EIF3H (5ʹ-GCAACTCTTGGAAGAAATATA-3ʹ) and (5ʹ-CCCAAGGATCTCTCTCACTAA-3ʹ) were gained from Ruibo Biotechnology Co., Ltd. (Guangzhou, China). Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) was utilized for plasmid and siRNA transfection according to the manufacturer's recommendations.
Ha k63
Manufactured by Addgene
The HA-K63 is a laboratory product available at Addgene. It is a type of lab equipment, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The core function of the HA-K63 is not available.
Automatically generated - may contain errors
Lab products found in correlation
Ha k48, by Addgene (4 mentions)
Ha ub, by Addgene (2 mentions)
Profection, by Promega (2 mentions)
Viafect, by Promega (2 mentions)
Flag keap1, by Addgene (2 mentions)
Gfp polyq74, by Addgene (2 mentions)
Gfp p62, by Addgene (2 mentions)
Gfp nrf2, by Addgene (2 mentions)
Gfp keap1, by Addgene (2 mentions)
Myc nrf2, by Addgene (2 mentions)
Flag nrf2, by Addgene (2 mentions)
Flag ulk1, by Addgene (2 mentions)
His k63, by Thermo Fisher Scientific (2 mentions)
Effectene, by Qiagen (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Small interfering rnas targeting, by GenePharma (1 mentions)
Sirna1, by GenePharma (1 mentions)
4 protocols using ha k63
1
Plasmid Transfection for EIF3H Study
2
Molecular Mechanisms of NRF2 Regulation
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GFP‐polyQ74 (#40262), GFP‐p62 (#38277), GFP‐NRF2 (#21549), GFP‐KEAP1 (#28025), Flag‐KEAP1 (#28023), Myc‐NRF2 (#21555), Flag‐NRF2 (#36971), HA‐K48 (#17605), HA‐K63 (#17606), Flag‐ULK1 (#27636) were procured from Addgene. GFP‐TRIM16 and Flag‐TRIM16 were cloned as described previously (Chauhan et al, 2016 ). Myc‐TRIM16, Myc‐TRIM16 deletion constructs, and His‐K63‐UB were generated using Gateway cloning strategy as per standard protocol (Invitrogen).
The siRNA for NRF2 and p62 were purchased from Sigma, and TRIM16, Ubb, Ube2n siRNA were from Dharmacon. For overexpression experiments, HEK293T cells were transfected using calcium phosphate method as per the manufacturer's instructions (Profection, Promega). Other cells are transfected using Effectene (Qiagen) or Viafect (Promega) or Interference (Polyplus) as per the manufacturer's instruction.
The siRNA for NRF2 and p62 were purchased from Sigma, and TRIM16, Ubb, Ube2n siRNA were from Dharmacon. For overexpression experiments, HEK293T cells were transfected using calcium phosphate method as per the manufacturer's instructions (Profection, Promega). Other cells are transfected using Effectene (Qiagen) or Viafect (Promega) or Interference (Polyplus) as per the manufacturer's instruction.
3
Plasmids and siRNAs for Ubiquitin Research
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The The full-length and deletion mutant constructs of TAZ and USP26 were obtained from Hanbio Biotechnology Co. Ltd. (Shanghai, China). The HA-K6, HA-K11, HA-K27, HA-K29, HA-K33, HA-K48, HA-K63, and HA-Ub plasmids were acquired from Addgene. Small interfering RNAs targeting USP26 (siRNA-1: 5′-GCACAAGACUUCCGUUGGA-3′; 5′-AAACAGAUCUGGUUCACUU-3′) were synthesized by Genepharma (Shanghai, China).
4
Characterization of NRF2-KEAP1 Pathway
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