All animal experimentations were performed in accordance with local ethical committees following the principles of laboratory animal care with the European Communities Council Directive 2010/63/UE. All efforts were made to minimize animal suffering and to reduce the number of animals used.
Novel object recognition and T-maze studies were conducted respectively in male OFA rats (150–175 g, Charles River, Saint-Germain-Nuelles, France) and male C57Bl/6 mice (5.5–6.5 month-old, Charles River, Saint-Germain-Nuelles, France). Animals were housed in collective cages with continuous access to tap water and food on a 12 h light-dark cycle, in a temperature-controlled (21 ± 1°C) and ventilated room. On completion of studies subjects were euthanized by either CO2 inhalation or intraperitoneal (i.p.) lethal pentobarbital injection. The novel object recognition test was used as the primary in vivo screening assay [32 ] to identify AMPA-PAMs. The T-maze task was performed using a modified continuous performance procedure previously described in mice [33 (link)].
Novel object recognition and T-maze studies were conducted respectively in male OFA rats (150–175 g, Charles River, Saint-Germain-Nuelles, France) and male C57Bl/6 mice (5.5–6.5 month-old, Charles River, Saint-Germain-Nuelles, France). Animals were housed in collective cages with continuous access to tap water and food on a 12 h light-dark cycle, in a temperature-controlled (21 ± 1°C) and ventilated room. On completion of studies subjects were euthanized by either CO2 inhalation or intraperitoneal (i.p.) lethal pentobarbital injection. The novel object recognition test was used as the primary in vivo screening assay [32 ] to identify AMPA-PAMs. The T-maze task was performed using a modified continuous performance procedure previously described in mice [33 (link)].