Sanford Research animal facility provided access to Vevo2100 ultrasound imaging system (FUJIFILM Visual Sonics, Toronto, Canada) equipped with MS400 and MS550D transducers (18–38 and 22–55 MHz); NYIT-COM provided access to Vivid 7 ultrasound instrument (GE Healthcare, Port Washington, NY) equipped with i13L transducer recommended for cardiac studies in rodents (5.9–14.1 MHz). Mice were anesthetized with 0.8–1.5% isoflurane and a parasternal short axis view was obtained in B-mode and recorded in M-mode. The M-mode echocardiograms were analyzed by tracing myocardial wall movement over 3–5 cardiac cycles using Vivo2100 image analysis software. Alternatively, left ventricular (LV) diameter, LV posterior and septal wall thicknesses in systole and diastole were recorded with time intervals for three cardiac cycles using EchoPac software (GE Healthcare, Port Washington, NY). Heart rate, ejection fraction, cardiac output, and LV mass were calculated using standard equations for rodents (as recommended by Visual Sonics).
I13l transducer
Manufactured by GE Healthcare
Sourced in Germany
The I13L transducer is a compact, linear array ultrasound transducer designed for general imaging applications. It features a wide frequency range and a large field of view to support a variety of clinical examinations.
Automatically generated - may contain errors
Lab products found in correlation
Vivid 7 ultrasound instrument, by GE Healthcare (2 mentions)
Vivid 7 dimension, by GE Healthcare (2 mentions)
Vevo 2100 ultrasound imaging system, by Fujifilm (1 mentions)
Echopac software, by GE Healthcare (1 mentions)
Echopac pc software, by GE Healthcare (1 mentions)
Vivid 7 dimension system, by GE Healthcare (1 mentions)
Ge vivid seven, by GE Healthcare (1 mentions)
6 protocols using i13l transducer
1
Cardiac Function Assessment in Mice
2
Echocardiographic Assessment of Pressure Overload
3
Echocardiographic Evaluation of Cardiac Function in Mice
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A Vivid 7 ultrasound instrument (GE Healthcare, Port Washington, NY) equipped with an i13L transducer (recommended for cardiac studies in rodents, 5.9‐14.1 MHz) was used in all experiments. Mice were anesthetized with 0.8% to 1.5% isoflurane, and a mid–left ventricular (LV) short‐axis view at the level of the papillary muscles was obtained in B‐mode and recorded in M‐mode. LV diameter, LV posterior, and septal wall thicknesses in systole and diastole as well as time intervals were measured and averaged over 3 cardiac cycles using EchoPAC PC software (GE Healthcare, Port Washington, NY). Heart rate, fractional shortening, ejection fraction, cardiac output, and LV mass were calculated using standard equations for rodents (as recommended by VisualSonics, Bothell, WA). Matched baseline and final echo data were available for 9 mice in the WHC+placebo group, 9 in the WHC+hArg group, 5 in WHC‐eTNAP+placebo group, and 6 in the WHC‐eTNAP+hArg group.
4
Echocardiography Evaluation of Cardiac Function
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Transthoracic echocardiography was performed before start of treatment and after 7 days of treatment, as described previously.23 (link) Mice were anesthetized using isoflurane inhalation (3% for induction and 2% for maintenance of anesthesia). M-Mode images and 2D parasternal short axis images were taken using a Vivid 7 Dimension (GE Healthcare, Munich, Germany) micro-imaging system equipped with an i13L transducer (14 MHz). Ejection fraction and LV geometry were quantified as described before.23 (link)
5
Transthoracic Echocardiography Post-MI
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Transthoracic echocardiography (TTE) studies were performed (VIVID 7 dimension system, General Electric-Vingmed Ultrasound) 15 d after MI surgery and cell implantation. Images were obtained using an i13L transducer (5.3-14.0 MHz, GE Healthcare) with high temporal and spatial resolution. Two-dimensionally targeted M-mode parameters were measured at a level of papillary muscle in parasternal short axis view during 6 consecutive cardiac beats. All measurements were performed in a blind fashion according to the guidelines of American Society for Echocardiography.
6
Ultrasound Imaging and Microbubble Detection
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The ultrasound examination was performed with a GE Vivid Seven (GE Medical Systems, Little Chalfont, UK). A high-frequency i13L transducer (5.7–11.4 MHz, GE Medical Systems) was used for B-mode and color Doppler imaging. The output power was set at − 15 dB, the mechanical index (MI) at 0.13, and the depth at 3 cm. The GE Vivid Seven ultrasound system with an M3S transducer (3.5 MHz, GE Medical Systems) was used for flashing the microbubbles. The mechanical index (MI) was adjusted according to the low-MI setting (0.08 MI with contrast pulse sequence) for imaging microbubbles of the femoral artery and high-MI (1.0) for diagnostic application. The transducer was fixed in a longitudinal view above a coupling medium tank 3–4 cm in depth so that the ultrasound beam could provide adequate coverage for the potential area of thrombosis. The rabbits were examined in the supine position.
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