Cobalt chloride

The human breast adenocarcinoma (MCF-7) cell line was obtained from the Egyptian Holding Company for Biological Products and Vaccines (VACSERA, Giza, Egypt). Ammonium nitrate, magnesium sulfate, potassium nitrate, boric acid, cobalt chloride, cupric sulfate, manganese sulfate, potassium iodine, sodium molybdate, zinc sulfate, citric acid and sodium citrate were purchased from Wako Pure Chemical Industries^® (Tokyo, Japan). Calcium chloride, dextrose, potassium hexacyanoferrate II, potassium hexacyanoferrate III and sodium carbonate were obtained from Merck^® (Darmstadt, Germany), while potassium phosphate, sodium chloride, ferrous sulfate, Na₂-EDTA2H₂O, nicotinic acid, pyridoxineHCl, thiamineHCl, Fe-EDTA, 4-morpholineethanesulfonic acid, τ-inositol, sucrose, phytoagar, sodium phosphate, silymarin, triton X-100, 5-bromo-4-chloro-3-indoly-β-d-glucuronide and α-Rhamnase were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA). 17β-estradiol was purchased from Takeda Chemical Industries Ltd. (Osaka, Japan). Solvents used were of analytical grade unless mentioned and were purchased from Merck, J.T. Backer^® (Deventer, The Netherlands) and Theo Seulberger^® (Karlsruhe, Germany). Media and supplements for cell cultures were obtained from Gibco^®/Invitrogen (Karlsruhe, Germany) and Greiner Labortechnik^® (Frickenhausen, Germany).

Nickel chloride (NiCl₂), zinc chloride (ZnCl₂), cobalt chloride (CoCl₂), copper (II) chloride dihydrate (CuCl₂·2H₂O), iron (II) chloride tetrahydrate (FeCl₂·4H₂O), magnesium chloride hexahydrate (MgCl₂·6H₂O), manganese (II) chloride tetrahydrate (MnCl₂·4H₂O), lipopolysaccharides (LPS) from Escherichia coli O111, and 30% (w/v) H₂O₂ were purchased from Wako Pure Chemical Industries (Osaka, Japan). Chlorazol Black and TAK-242 were purchased from Sigma-Aldrich Co. (St. Louis, MO) and Calbiochem-Merck Millipore (Darmstadt, Germany), respectively. Newport Green^TM DCF diacetate was purchased from Invitrogen (Carlsbad, CA) and the Ni wire (purity 99.98%, diameter 0.8 mm) from Nilako (Tokyo, Japan). HNO₃ (69% (w/w)) was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan).