Pyrrole (Py; 98%), Pyrrole-2-carboxylic acid (P3CA, 99%), sodium bis(2-ethylhexyl)-sulfosuccinate (AOT; 96%), FeCl3 (97%), hexane (>95%), (3-aminopropyl)triethoxysilane (APTES; >98%), and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM; >96%) were purchased from Sigma-Aldrich (MO, USA) and used without further purification. Cortisol (human; >98%), cortisone, prednisolone, and corticosterone were also purchased from Sigma-Aldrich. Mouse monoclonal anti-Cortisol IgG was purchased from Abcam (CORT-2, Cat. No. ab1952, UK). Stock solutions of Cortisol and its analogs were prepared in phosphate-buffered saline at 270 µM and stored at −20 °C. Working solutions were freshly prepared by dilution of the stock solution.
Sodium bis 2 ethylhexyl sulfosuccinate
Manufactured by Merck Group
Sourced in United States
Sodium bis(2-ethylhexyl)-sulfosuccinate is a chemical compound used in various laboratory applications. It functions as a surfactant, helping to reduce surface tension and facilitate interactions between different substances. The core purpose of this product is to enable efficient mixing, solubilization, and emulsification in various laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Cortisone, by Merck Group (1 mentions)
Cortisol, by Merck Group (1 mentions)
Pyrrole, by Merck Group (1 mentions)
Prednisolone, by Merck Group (1 mentions)
3 aminopropyltriethoxysilane, by Merck Group (1 mentions)
Pyrrole 2 carboxylic acid, by Merck Group (1 mentions)
4 4 6 dimethoxy 1 3 5 triazin 2 yl 4 methylmorpholinium chloride dmt mm, by Merck Group (1 mentions)
Fecl3, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
Corticosterone, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Nh4oh 25, by Merck Group (1 mentions)
C14h14n3nao3s, by Merck Group (1 mentions)
Hcl 35 suprapure, by Merck Group (1 mentions)
Methyl orange, by Merck Group (1 mentions)
Ethyl alcohol, by Merck Group (1 mentions)
4 protocols using sodium bis 2 ethylhexyl sulfosuccinate
1
Cortisol Immunoassay Protocol
2
Laccase-Mediated Antioxidant Assay
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Laccase from Trametes versicolor (TvL, EC 1.10.3.2; product no. 51639, 13.6 U mg−1, lot no. BCBF7247 V), sodium bis(2-ethylhexyl) sulfosuccinate (AOT ≥ 99%), sodium phosphate monobasic (NaH2PO4), chloroform (ReagantPlus ≥ 99.8%, 0.5-1.0% ethanol as stabilizer), 2,2-Diphenyl-1-picrylhydrazyl (DPPH, 95%), and N-phenyl-1,4-phenylendiamine (=p-aminodiphenylamine, PADPA, 98%) were purchased from Sigma-Aldrich. PADPA was purified by multiple recrystallizations from hexane until white crystals were isolated. Analytical grade ethanol was purchased from Scharlau. Phosporic acid (H3PO4, 85%) and Methyl-tert-butyl ether (MTBE, ≥99.0%) were bought from Fluka. All aqueous solutions were prepared with Milli-Q water.
3
Sensitive Colorimetric Assay Development
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1-Aminopropyl-3-methyl-imidazolium bromide ([APMIm][Br], ≥99 wt%) was obtained from Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (China). Citric acid monohydrate (CA, ≥99.5 wt%), thiourea (≥99 wt%), potassium dihydrogen phosphate (≥99 wt%) and disodium hydrogen phosphate (≥99 wt%) were purchased from Shanghai Runjie Chemical Reagent Co., Ltd. Sodium bis(2-ethylhexyl) sulfosuccinate (AOT, >99% wt%) was purchased from Sigma-Aldrich Chemical Co. 3,3′,5,5′-Tetramethylbenzidine (TMB, 98 wt%), ethanol (99 wt%), and tert-butanol (TBA, 99 wt%) were supplied by Aladdin. Hydrogen peroxide (H2O2, 30 wt%) was obtained from Chinasun Specialty Products Co., Ltd All reagents were used directly without further purification, and all aqueous solutions were prepared with ultrapure water (18.2 MΩ) from a ultra-pure water system (DZG-303A, Leading Water Treatment Co. Ltd, Shanghai).
4
Cobalt Ferrite Preparation and Dye Adsorption
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The cobalt ferrite samples were prepared by the use of Fe(NO3)3·9H2O 99%, Co(NO3)2·6H2O and sodium bis-2-ethyl-hexyl sulfosuccinate (analytical grade, Sigma Aldrich, St. Louis, MO, USA). NH4OH 25% and HCl 35% suprapure (analytical grade, Merck KGaA Germany, Darmstadt, Germany) were used to adjust the solution’s pH. The dye solutions of desired concentrations were obtained from Congo Red (CR) 99% (C32H22N6Na2O6S2) and Methyl Orange (MO) (ACS reagent, Dye content 85%, C14H14N3NaO3S, Sigma Aldrich). Ethyl alcohol (95%) (Sigma Aldrich, St. Louis, MO, USA) was applied as a desorbing agent. Ammonium acetate 99% and acetonitrile 99% (Merck KGaA Germany, Darmstadt, Germany) were utilized as a mobile phase for the dye’s chromatographic separation and detection.
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