5 heneicosyl resorcinol

Manufactured by Merck Group

5-heneicosyl-resorcinol is a laboratory reagent used for research purposes. It is a phenolic compound with a long aliphatic side chain. The core function of this product is to serve as a building block or intermediate in various chemical synthesis and analysis applications. Further details on its intended use are not available.

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Resorcinol (135 citations)

3 protocols using 5 heneicosyl resorcinol

Resorcinol Derivatives Analysis Protocol

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5-Nonadecyl-resorcinol, 5-heneicosyl-resorcinol,
5-tricosyl-resorcinol, 5-heptadecylresorcinol (10 mg powder), 2,5-dihydroxybenzoic
acid (DHB), and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich
(Steinheim). Gelatin used for embedding was obtained from VWR International
(Darmstadt, Germany). Glass microscope slides (ground edges, super
frost) were obtained from R. Langenbrinck (Emmendingen, Germany).
LC–MS-grade methanol, ethyl acetate, and 2-propanol were purchased
from Scharlab Italia Srl (Milan, Italy); bidistilled water was obtained
using a Milli-Q system (Millipore, Bedford, MA, USA). MS-grade ammonium
formate and formic acid from Fisher Chemical (Thermo Fisher Scientific,
Inc., San Jose, CA, USA) were also used.

Pedrazzani C., Vanara F., Bhandari D.R., Bruni R., Spengler B., Blandino M, & Righetti L. (2021). 5-n-Alkylresorcinol Profiles in Different Cultivars of Einkorn, Emmer, Spelt, Common Wheat, and Tritordeum. Journal of Agricultural and Food Chemistry, 69(47), 14092-14102.

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Analytical Standards for Mycotoxin Detection

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5-Nonadecyl-resorcinol, 5-heneicosylresorcinol,
5-tricosyl-resorcinol, 5-heptadecylresorcinol (10 mg powder), 2,5-dihydroxybenzoic
acid (DHB), and phenolic acid standards (caffeic acid ≥98%, p-coumaric acid ≥98%, ferulic acid ≥99%, gallic
acid ≥99%, protocatechuic acid ≥99%, p-hydroxybenzoic acid ≥99%, sinapic acid ≥98%, syringic
acid ≥95%, and vanillic acid ≥97%) were purchased from
Sigma-Aldrich (Steinheim). Analytical standards of DON (100 mg L^–1 in acetonitrile) and deoxynivalenol-3-glucoside (DON3Glc)
(50.6 mg L^–1 in acetonitrile) were purchased from
Romer Laboratories (Tulln, Austria).
LC–MS grade methanol,
ethyl acetate, and 2-propanol were purchased from Scharlab Italia
Srl (Milan, Italy); bidistilled water was obtained using a Milli-Q
system (Millipore, Bedford, MA, USA). MS-grade ammonium formate and
formic acid from Fisher Chemical (Thermo Fisher Scientific, Inc.,
San Jose, CA, USA) were also used.

Righetti L., Vanara F., Bruni R., Sardella C., Blandino M, & Dall’Asta C. (2024). Investigating Metabolic Plant Response toward Deoxynivalenol Accumulation in Four Winter Cereals. Journal of Agricultural and Food Chemistry, 72(6), 3200-3209.

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Comprehensive Metabolite Identification Protocol

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The fidelity of chemical identification was defined at four levels according to the published guidelines for metabolomics [22] .
3-Carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF), 3,5ihydroxybenzoic acid (3,5-DHBA), and 3,5dihydroxyphenylpropionic acid (3,5-DHPPA) were annotated using an in-house database, which includes retention time and MS/ MS spectra of authentic standards. Some of the lysophosphatidylcholines (LPCs) and phosphatidylcholines (PCs) that were not present in the in-house standard database were identified using a previously described Matlab function [23] . The authentic standards, trans-2-octenoylcarnitine (CN(C8:1)), 5-Heneicosylresorcinol (AR(C21:0)), nonadecylresorcinol (AR(C19:0)), and human liver S9 and microsomes were purchased from Sigma Aldrich. MS/MS spectra were compared with comparable spectra in available databases, such as METLIN, HMDB, mzCloud and produced by in-silico fragmentation tools such as CFM-ID, FingerID and lipidmaps. Glucuronide conjugates of CMPF, AR(C19:0), AR(C21:1), 3,5-DHBA and 3,5-DHPPA were produced using human liver S9 as described previously [24] . CMHPF, a hydroxylated metabolite of CMPF, was produced by incubating CMPF with liver microsomes [25, 26] and the identity confirmed as described in Supplementary Fig. 2, whereas the annotation CPF and CMPentylF was based on MS/MS matches with CMPF.

Gürdeniz G., Uusitupa M., Hermansen K., Savolainen M.J., Schwab U., Kolehmainen M., Brader L., Cloetens L., Herzig K.H., Hukkanen J., Rosqvist F., Ulven S.M., Gunnarsdóttir I., Thorsdottir I., Oresic M., Poutanen K.S., Risérus U., Åkesson B, & Dragsted L.O. (2022). Analysis of the SYSDIET Healthy Nordic Diet randomized trial based on metabolic profiling reveal beneficial effects on glucose metabolism and blood lipids. Clinical nutrition (Edinburgh, Scotland), 41(2).

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