Production or consumption of glucose, fructose, glycerol, acetic acid and ethanol, were determined by HPLC in duplicate, using a Surveyor Plus chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and a photodiode array detector (Surveyor RI Plus and Surveyor PDA Plus, respectively). A Hyper REZ XP carbohydrate H+ 8 μm column and guard (Thermo Fisher Scientific) were used and maintained at 50 °C. Elution was performed with 1.5 mM H2SO4 as mobile phase, at a flow rate of 0.6 mL/min. Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
Hyper rez xp carbohydrate h 8 μm column and guard
Manufactured by Thermo Fisher Scientific
The Hyper REZ XP carbohydrate H+ 8 μm column and guard is a liquid chromatography column designed for the analysis of carbohydrates. The column has a particle size of 8 micrometers and utilizes a strong cation exchange resin for the separation of carbohydrates.
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2 protocols using hyper rez xp carbohydrate h 8 μm column and guard
1
HPLC Analysis of Metabolites
2
Quantitative Analysis of Fermentation Metabolites
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Production and consumption of the main fermentation-related metabolites in daily samples, (glucose, fructose, glycerol, acetic acid and ethanol) were determined in duplicate using a Surveyor Plus chromatograph (Thermo Fisher Scientific, Waltham, MA) equipped with a refractive index and a photodiode array detector (Surveyor RI Plus and Surveyor PDA Plus, respectively). Hyper REZ XP carbohydrate H+8 μm column and guard (Thermo Fisher Scientific) were used and maintained at 50°C. Elution was performed with 1.5 mM H 2 SO 4 as mobile phase, at a flow rate of 0.6 mL/min.
Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
One way analysis of variance was carried out on the main fermentation metabolites. Means of biological replicates were compared using Tukey's test, with significance level set at 5%. All analyses were performed using SPSS Statistics v. 23 program (IBM, Armonk, NY).
Prior to injection, samples were filtered through 0.22-μm-pore-size nylon filters and diluted 10-fold.
One way analysis of variance was carried out on the main fermentation metabolites. Means of biological replicates were compared using Tukey's test, with significance level set at 5%. All analyses were performed using SPSS Statistics v. 23 program (IBM, Armonk, NY).
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