Prominence uflc xr

Manufactured by Shimadzu

Sourced in Japan

The Prominence UFLC XR is an ultra-fast liquid chromatography (UFLC) system developed by Shimadzu. It is designed to provide high-speed and high-resolution analysis of samples. The system features advanced components and technologies to enable efficient and reliable separation and detection of chemical compounds.

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Lab products found in correlation

Qtrap 5500, by AB Sciex (2 mentions) Cadenza cd c18 column, by Imtakt (1 mentions) Absoluteidq p180 kit, by Biocrates (1 mentions) Metiq software, by Biocrates (1 mentions) Brownlee spp c18 column, by PerkinElmer (1 mentions) Qtrap 5500 mass spectrometer, by AB Sciex (1 mentions) Symmetry reverse phase c 18 column, by Waters Corporation (1 mentions) Ascentis express rp amide analytical column, by Merck Group (1 mentions) 4000 qtrap, by AB Sciex (1 mentions) Spd m20a, by Shimadzu (1 mentions) Poroshell 120 ec c18 column, by Agilent Technologies (1 mentions) Acetonitrile, by Merck Group (1 mentions) Beh c18 column, by Waters Corporation (1 mentions)

Spelling variants of this product

Prominence UFLC (46 citations) UFLC XR (30 citations) Prominence UFLC-XR system (10 citations) Prominence UFLC XR liquid chromatography system (2 citations) Prominence UFLC XR 20 (2 citations)

10 protocols using prominence uflc xr

LC-MS Analysis of Reaction Mixtures

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LC-MS was carried out using a Prominence UFLC-XR (Shimadzu, Kyoto, Japan) equipped with a Cadenza CD-C18 column (Imtakt, Kyoto, Japan) and an ESI-MS detector micrOTOF II-kp (Bruker, Massachusetts, United States). After the labeling reaction, 10 μL of the mixture was injected and monitored. The reactants were eluted using 0.1% formic acid/acetonitrile solutions with a flow rate of 0.2 mL/min. The absorbance of the eluate was monitored at 210 nm, and mass analysis was done in a negative ion mode.

Sano M., Kuroda H., Ohara H., Ando H., Matsumoto K, & Aso Y. (2019). A high-throughput screening method based on the Mizoroki-Heck reaction for isolating itaconic acid-producing fungi from soils. Heliyon, 5(7), e02048.

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Targeted Metabolomic Analysis of Jejunal Citrulline

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Jejunal citrulline was quantified via liquid chromatography tandem mass spectrometry (LC-MS/MS) using Biocrates AbsoluteIDQ p180 kit (BIOCRATES, Life Science AG, Innsbruck, Austria). The LC-MS/MS platform consisted of a Shimadzu Prominence UFLC XR high-performance liquid chromatograph (HPLC) (Shimadzu, Columbia, MD) coupled to an AB Sciex QTRAP^® 5500 hybrid tandem quadrupole/linear ion trap mass spectrometer (AB Sciex, Framingham, MA). The AbsoluteIDQ p180 kit was designed for simultaneous detection and quantification of metabolites from a variety of biological matrices in a high-throughput manner and referred to as high-throughput, targeted metabolomics. The AbsoluteIDQ p180 kit was prepared as described by the manufacturer. Briefly, the kit preparation involved metabolite extraction from the tissue samples by homogenizing in 85:15 (methanol:ethanol, v/v) with 5 mM PBS followed by introduction of stable-label isotope internal standards, amino acid derivatization with 5 % phenylisothiocyanate, and extraction of the metabolites with 5 mM ammonium acetate in methanol on filter inserts of a 96-well plate. Data was analyzed using the MetIQ software (Biocrates, Inc.). Although all metabolites from the AbsoluteIDQ p180 kit were analyzed for, only the citrulline values are presented in this report.

Jones J.W., Tudor G., Li F., Tong Y., Katz B., Farese A.M., MacVittie T.J., Booth C, & Kane M.A. (2015). Citrulline as a biomarker in the murine total-body irradiation model: correlation of circulating and tissue citrulline to small intestine epithelial histopathology. Health physics, 109(5), 452-465.

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Plasma Creatinine Measurement in NHPs

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Blood was taken by saphenous vein venipuncture under ketamine anesthesia just before euthanasia. BUN and serum creatinine were determined using commercial kits on an Alfa Wassermann Ace Clinical Chemistry Analyzer (4 Henderson Dr., West Cladwell, NJ), the latter using the Jaffé reaction. Plasma creatinine was confirmed by liquid chromatography - tandem mass spectrometry (LC-MS/MS) in a subset of 53 animals using the Biocrates AbsoluteIDQ p180 kit (Biocrates, Life Science AG, Innsbruck, Austria) on a Shimadzu Prominence UFLC XR high-performance liquid chromatograph (HPLC) (Shimadzu, Columbia, MD) coupled to an AB Sciex QTRAP® 5500 hybrid tandem quadrupole/linear ion trap mass spectrometer (AB Sciex, Framingham, MA). Normal values were confirmed using blood samples from age-matched non-irradiated NHP.

Cohen E.P., Hankey K.G., Farese A.M., Parker G.A., Jones J.W., Kane M.A., Bennett A, & MacVittie T.J. (2019). Radiation nephropathy in a nonhuman primate model of partial-body irradiation with minimal bone marrow sparing. Part 1: acute and chronic kidney injury and the influence of Neupogen. Health physics, 116(3), 401-408.

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Quantitative Analysis of Chlorophyll a and b

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The green pigment was extracted according to the previously described method, and quantitative analyses for Chl a and Chl b were performed using Prominence UFLC XR (Shimadzu, Kyoto, Japan) with Brownlee SPP C18 column (4.6 × 100 mm, 2.7 um, Perkinelmer, Waltham, MA, USA). The mobile phases were separated using an elution gradient, (A) acetone/methanol (1:4, v/v) and (B) ion pair reagent/methanol (1:4, v/v), at a flow rate of 0.6 mL/min. The ion pair reagent was 1 M ammonium acetate in water; the gradient was isocratic A for 4 min, A to B for 5 min, isocratic B for 4 min, and again isocratic A for 2 min. The injected sample (5 μL) was monitored at a wavelength of 650 nm.

Shin N.H., Trang D.T., Hong W.J., Kang K., Chuluuntsetseg J., Moon J.K., Yoo Y.H., Jung K.H, & Yoo S.C. (2019). Rice Senescence-Induced Receptor-Like Kinase (OsSRLK) Is Involved in Phytohormone-Mediated Chlorophyll Degradation. International Journal of Molecular Sciences, 21(1), 260.

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Targeted Eicosanoid Profiling by LC-MS/MS

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LC separation was performed with a Shimadzu Prominence UFLC XR (Shimadzu Corporation). The multiple-reaction-monitoring (MRM) spectra were obtained with a QTRAP 5500 mass spectrometer (AB SCIEX) equipped with an ESI source. A Waters Symmetry reverse-phase C18 column (2.1 mm × 150 mm, 3.5 μm) was used for the LC separation as described (Meng et al., 2016 (link)). Up to 35 eicosanoids and two internal standards [15(S)-HETE-d₈ and PGB₂] were monitored. Optimized LC-MS/MS parameters for the analysis of eicosanoids are in accordance with previous research (Meng et al., 2016 (link)).

Li C., Deng X., Xie X., Liu Y., Friedmann Angeli J.P, & Lai L. (2018). Activation of Glutathione Peroxidase 4 as a Novel Anti-inflammatory Strategy. Frontiers in Pharmacology, 9, 1120.

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HPLC Separation of Retinoic Acid Isomers

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Two different gradients were developed to resolve RA and its isomers. A shorter gradient (gradient 1) was mainly used for cultured cells or subcellular fractions and a longer gradient (gradient 2) was used primarily for plasma and tissue samples. Both gradients were performed using a Shimadzu Prominence UFLC XR high-performance liquid chromatograph (HPLC) (Shimadzu, Columbia, MD). All separations were performed using an Ascentis Express RP-Amide guard cartridge column (Supelco, 50 × 2.1 mm, 2.7 μm) coupled to an Ascentis Express RP-Amide analytical column (Supelco, 100 × 2.1 mm, 2.7 μm). Mobile phase A consisted of 0.1% formic acid in water, and mobile phase B consisted of 0.1% formic acid in acetonitrile. Refer to the Supporting Information for a description of gradients and LC operating parameters.

Jones J.W., Pierzchalski K., Yu J, & Kane M.A. (2015). Use of Fast HPLC Multiple Reaction Monitoring Cubed for Endogenous Retinoic Acid Quantification in Complex Matrices. Analytical chemistry, 87(6), 3222-3230.

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Comprehensive Chromatographic Detection of Palytoxin and Analogues

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Liquid chromatography was carried out on a Poroshell 120 EC-C18 column (100 × 2.1 mm, 2.7 μm, Agilent) equipped with a guard column (5 × 2.1 mm, 2.7 μm, using the same stationary phase). An Ultra-Fast Liquid Chromatography system (Prominence UFLC-XR, Shimadzu) was employed for this purpose. Gradients of water (A) and acetonitrile (95%, B), both containing 0.2% acetic acid, were utilized at a flow rate of 0.2 mL min⁻¹. The injection volume was 5 μL, and the column temperature was maintained at 25 °C. MS/MS analyses were conducted using a 4000 QTRAP (AB Sciex) in positive ion mode, employing multiple reactions monitoring (MRM) acquisition. UV detection at 220 nm, 233 nm, 263 nm and in the range of 220–360 nm was performed with a diode array detector (Prominence, SPD-M20A, Shimadzu). In total, two LC–MS/MS methods and one LC–UV–MS/MS method, as described in Chomérat [45 (link)], were employed to detect palytoxin, 42-OH-palytoxin, 12 ovatoxins (OVTX-a to -k), 3 ostreocins (OST-A, -B, -D and -E1), 3 mascarenotoxins (McTX-A to -C) and ostreotoxins-1 and -3. Quantification was carried out relative to a Palytoxin standard (Wako Chemicals GmbH, Germany) using a 6-point calibration curve.

Ibghi M., Rijal Leblad B., L’Bachir El Kbiach M., Aboualaalaa H., Daoudi M., Masseret E., Le Floc’h E., Hervé F., Bilien G., Chomerat N., Amzil Z, & Laabir M. (2024). Molecular Phylogeny, Morphology, Growth and Toxicity of Three Benthic Dinoflagellates Ostreopsis sp. 9, Prorocentrum lima and Coolia monotis Developing in Strait of Gibraltar, Southwestern Mediterranean. Toxins, 16(1), 49.

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Separation of Sudan Dyes by LC-DAD-MS/MS

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LC was performed using a Prominence UFLC XR (Shimadzu, Kyoto, Japan). Isocratic elution was used to separate the Sudan dyes (I–IV) with an Agilent Eclipse 5 μm XDB-C18 (4.6 mm × 150 mm) column at 40°C and a flow rate of 0.8 ml min⁻¹ for 30 min. For LC separation with DAD detection, a 150 mm column along with a flow rate of at least 0.5 ml min⁻¹ is needed for good separation between the natural colours and Sudan dyes. For analysis using MS/MS without the DAD detector in series, a shorter column with a smaller particle size and lower flow rate would reduce solvent and the run time and maximise sample throughput. The mobile phase was 95/5 v/v methanol: water buffered with 5 mM ammonium formate and 0.1% formic acid with an injection volume of 5 μl. A 5-mincolumn wash was added at the end of each run using 98/2 v/v isopropanol:water solution buffered with 2 mM ammonium formate and 0.03% formic acid followed by a 5-minequilibration with mobile phase solvents. Additionally, during DAD analysis, a wash of 100% isopropanol was added at the end of each sample set to remove the buffered mobile phase from the DAD detector. The LC conditions (flow rate, column selection) were optimised for use with a DAD and MS/MS detector in series on the same instrument in order to develop a method that could be easily transferred among instruments with different detectors for testing adulterated samples.

Genualdi S., MacMahon S., Robbins K., Farris S., Shyong N, & DeJager L. (2016). Method development and survey of Sudan I–IV in palm oil and chilli spices in the Washington, DC, area. Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 33(4), 583-591.

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Quantitative Analysis of Atrazine and Metabolites

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Atrazine and its main metabolites (deethylatrazine, DEA, and deisopropylatrazine, DIA)
were determined in soil and plant samples by HPLC, analysing the extracts obtained by a shaking-centrifuging extraction procedure (Amadori et al., 2013) . A sample of 2.0 g of soil or plant tissues (previously chopped, crushed and blended) was put in a glass flask;
3 mL of acetonitrile (Sigma-Aldrich, USA) were added and the mixture was shaken for 30 min and centrifuged for 15 min. This procedure was sequentially executed three times and the respective supernatant phases were combined. 0.45 µm nylon syringe filters were used to filtrate the resulting extracts before the HPLC determination. A Shimadzu Prominence UFLC XR (Japan) chromatograph equipped with a C18 reversephase column (240 mm x 4 mm) and a diode array detector was used to separate ATR, DEA and DIA. The details of the analytical method used here have been previously
reported (Sánchez et al., 2018) (link). ATR, DEA and DIA standard solutions were prepared from the corresponding solid reagents (Sigma-Aldrich, USA).

Sánchez V., F.r.a.n.c.i.s.c.o., López-Bellido J., Rodrigo M.A, & Rodríguez L. (2019). Enhancing the removal of atrazine from soils by electrokinetic-assisted phytoremediation using ryegrass (Lolium perenne L.). Chemosphere, 232.

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Lipidomic Analysis by LC-MS/MS

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The LC-MS/MS analysis was performed using a Prominence UFLC-XR (Shimadzu, Columbia, MD) followed by a quadrupole time-of-flight mass spectrometer (AB Sciex 5600, Sciex, Framingham, MA). The analysis of PL (PC and SM) was done by injecting 5 µL of each extracted sample onto a BEH C18 column (100 × 2.1 mm, 1.7 µM; Waters, Milford, MA). The mobile phase comprised solvent A (60% acetonitrile with 40% water, 10 mM ammonium formate, 0.1% formic acid) and solvent B (90% isopropanol, 10% acetonitrile, 10 mM ammonium acetate, 0.1% formic acid). A gradient dilution was used for 20 min as follows: 40% B was held for 0:01 min:s, increased to 43% B at 2:00 min:s, increased to 50% B at 2:10 min:s, increased to 54% B at 12:00 min:s, increased to 70% B at 12:10 min:s, increased to 99% B at 18:00 min:s, held at 99% B up to 20 min, and decreased to 40% B at 20:10 min:s with a flow rate of 0.225 mL/min. The analysis for PC and SM was performed in positive mode. The positive ion electrospray ionization mass spectra were obtained over a mass range of 100 to 1,200 mass: charge ratio (m/z) in an information-dependent acquisition mode with one 100-ms survey scan and up to twenty 100-ms MS/MS product ion scans per duty cycle. Three determinations were done for each replicate.

Cheema M., Smith P.B., Patterson A.D., Hristov A, & Harte F.M. (2018). The association of lipophilic phospholipids with native bovine casein micelles in skim milk: Effect of lactation stage and casein micelle size. Journal of dairy science, 101(10).

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