Elastase 1

Immunoblotting and immunoprecipitation were performed as previously described (65 (link)). Equal amounts of tissue lysates were analyzed, and the blots were incubated with specific antibodies to elastase 1 (Sigma-Aldrich), elastase 2 (Abgent), kallikrein 1 (Sigma-Aldrich), kallikrein 2 (Abgent), kallikrein 5 (Acris Antibodies), kallikrein 6 (Sigma-Aldrich), and collagen I, II, III, IV, fibronectin, fibrinogen and laminin (all from Abcam) as previously described (65 (link)). Beta-Actin (Sigma-Aldrich) was used as a loading control.

Immunoblotting was performed as previously described [25 (link)]. Equal amounts of cellular protein or tissue lysates were used. Blots were incubated with specific antibodies to elastase 1, kallikrein 1 and 6 (all 200 ng/ml; Sigma-Aldrich), elastase 2 (200 ng/ml; Abgent), kallikrein 2 (300 ng/ml; Abgent), kallikrein 5 (300 ng/ml; Acris Antibodies), c-kit (200 ng/ml; Cell Signaling Technology), Sca1 (200 ng/ml; Merck Millipore), CD10 (1:100; ThermoFisher), CD44, CD90 (both 200 ng/ml; Abcam), CD71 (1:200; ThermoFisher), pSMAD1/5/8, Sox2, Klf4, Slug (all 400 ng/ml; Cell Signaling Technology) or total SMAD (400 ng/ml; Santa Cruz Biotechnology). ß-Actin (1:5000 dilution; Sigma-Aldrich) was used as loading control.