Anti human cd33 clone wm 53

NOD-SCID IL-2Rγc deficient (NSG) mice were purchased from Charles River France or bred in-house and maintained under specific pathogen-free conditions. Mice maintenance and experimental procedures were performed in accordance with protocols approved and compliance with policies approved by the local Committee for Animal Experimentation of Marseille (CAE of Provence number 14), France (2-091009). Healthy 6- to 8-week-old male mice were sublethally irradiated (1.5 Gy) on D0 and injected i.v. with 1.10⁶ PBMCs from AML patient depleted for CD3⁺ T cells (depletion kit, MACS^® Technology, Miltenyi Biotec). When AML blasts were detected by cytometry in blood, mice were randomly assigned to receive four daily injections i.v. of pomalidomide (5 mg/kg in 100 µL of PBS) or control (PBS + DMSO). Progression of leukemia was evaluated in blood by flow cytometry using appropriated mAbs [anti-mouse CD45 (clone 30-F11, eBiosciences), anti-human CD45 (clone J.33, Beckman Coulter), anti-human CD33 (clone WM53, BD Biosciences)], and LIVE/DEAD Fixable Dead Cell Stain Kit for viability (Life Technologies). Number of hCD45⁺ cells per microliter of blood was quantified by flow cytometry using quantification beads (CountBright^TM absolute counting beads, Life Technologies).