Flow cytometric analysis was performed in accordance with previously described methods [15 (link)]. An anti-human CD44 IgG antibody (BioLegend, San Diego, CA, 338804, 1:20), anti-human CD73 IgG antibody (BioLegend, 344004, 1:20), anti-human CD90 IgG antibody (BioLegend, 328108, 1:20), anti-human CD11b IgG antibody (BioLegend, 301404, 1:20), anti-human CD34 IgG antibody (BioLegend, 343504, 1:20), anti-human CD45 IgG antibody (BioLegend, 304006, 1:20), anti-human HLA-A–C IgG antibody (BioLegend, 311404, 1:20), and anti-human HLA-DR IgG antibody (BioLegend, 307604, 1:20) were used. Stained ASCs were analyzed by a BD FACSVerse (Becton, Dickinson and Company, Franklin Lakes, NJ). The data were evaluated by FlowJo software (FlowJo, LLC, Ashland, OR).
Anti human cd44 igg antibody
Manufactured by BioLegend
Sourced in United States
The Anti-human CD44 IgG antibody is a laboratory reagent used for the detection and analysis of the CD44 protein, which is a cell surface glycoprotein involved in cell-cell interactions, cell adhesion, and migration. This antibody can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and quantify CD44-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Facsverse, by BD (3 mentions)
Anti human cd73 igg antibody, by BioLegend (3 mentions)
Anti human cd90 igg antibody, by BioLegend (3 mentions)
Anti human cd11b igg antibody, by BioLegend (3 mentions)
Anti human cd34 igg antibody, by BioLegend (3 mentions)
Anti human cd45 igg antibody, by BioLegend (3 mentions)
Anti human hla dr igg antibody, by BioLegend (3 mentions)
Anti human cd29 igg antibody, by BioLegend (2 mentions)
3 protocols using anti human cd44 igg antibody
1
Immunophenotyping of Adipose-Derived Stem Cells
2
Flow Cytometry Characterization of MSCs
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Flow cytometric analysis was performed in accordance with previously described methods [25 (link)]. The following antibodies were used: anti-human CD29 IgG antibody (BioLegend, San Diego, CA), anti-human CD44 IgG antibody (BioLegend), anti-human CD73 IgG antibody (BioLegend), anti-human CD90 IgG antibody (BioLegend), anti-human CD11b IgG antibody (BioLegend), anti-human CD34 IgG antibody (BioLegend), anti-human CD45 IgG antibody (BioLegend), anti-human HLA-A,B,C IgG antibody (BioLegend), and anti-human HLA-DR IgG antibody (BioLegend). The stained MSCs were analyzed by a BD FACSVerse (Becton, Dickinson and Company, Franklin Lakes, NJ). Data were assessed by FlowJo software (FlowJo, LLC; Ashland, OR).
3
Flow Cytometric Analysis of Mesenchymal Stem Cells
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Flow cytometric analysis was performed according to previously described methods [26 (link)]. The following antibodies were used: anti-human CD29 IgG antibody (BioLegend, San Diego, CA, USA), anti-human CD44 IgG antibody (BioLegend), anti-human CD73 IgG antibody (BioLegend), anti-human CD90 IgG antibody (BioLegend), anti-human CD11b IgG antibody (BioLegend), anti-human CD34 IgG antibody (BioLegend), anti-human CD45 IgG antibody (BioLegend), anti-human HLA-A,B,C IgG antibody (BioLegend), and anti-human HLA-DR IgG antibody (BioLegend). The stained MSCs were analyzed using a BD FACSVerse (Becton, Dickinson and Company, Franklin Lakes, NJ, USA). Data were assessed by FlowJo software (FlowJo, LLC; Ashland, OR, USA).
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