EL4 cells (catalog number: 87020408) were purchased from Sigma. Murine CD19 was transduced into EL4 via viral transduction. Phoenix-ECO (catalog number: CRL-3214) and A20 cells (TIB-208) were purchased from ATCC. A20-MHC I and MHCII knockout lines were generated using CRISPR as described (12 (link)). Tumor cells were maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1% penicillin/streptomycin. Retroviral producing cell lines (Phoenix-ECO and 293 Glv9) were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine and 1% penicillin/streptomycin. All cell lines are regularly tested for mycoplasma contamination.
A20 cells tib 208
Manufactured by American Type Culture Collection
Sourced in United States
A20 cells (TIB-208) are a well-established mouse B cell lymphoma cell line. They are routinely used in immunology research and assays.
Automatically generated - may contain errors
Lab products found in correlation
El4 cells, by Merck Group (2 mentions)
Phoenix eco, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by American Type Culture Collection (1 mentions)
2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 protocols using a20 cells tib 208
1
Generation of Murine Tumor Cell Lines
2
Generation of Murine Tumor Cell Lines
3
Enzymatic Substrate Cleavage Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SPPL2a, SPPL2b, SPP, and chimeric substrate FBA were generated as previously described (Ran et al, 2015 ). A20 cells (TIB‐208; ATCC, Manassas, VA, USA) were cultured in RPMI‐1640 medium (ATCC) supplied with 10% fetal bovine serum, 1% penicillin/streptomycin, and 0.05 mM 2‐mercaptoethanol (Life Technologies). Various concentrations of GSIs and (ZLL)2‐ketone as indicated in figures were applied to transfected HEK 293t cells at 70–90% confluence or A20 cells at a density of 5 × 105 cells/ml with fresh media in 48‐well format for 16 h. Cells and conditioned media were used for WB, IP, and ELISA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!