B7 1 and mcp 1

Cultured cells were lysed and isolated renal glomeruli were homogenized in RIPA buffer supplemented with protease inhibitors. Nuclear fractions of cultured cells or isolated glomeruli were prepared by using the NE-PER kit (Thermo Scientific, Rockford, Illinois, USA). Samples were subjected to immunoblot analysis as previously described.^{28 (link)} The antibodies against GSK3β, p-GSK3β, synaptopodin, cathepsin L, GAPDH were purchased from Santa Cruz Biotechnology and those against p-RelA/p65 serine 467, p-RelA/p65 serine 536, p-RelA/p65 serine 276, cleaved caspase 3, β-catenin, histone H3 were purchased from Cell Signaling (Beverly, MA, USA). The antibodies against B7-1 and MCP-1 was purchased from R&D Systems (Minneapolis, Minnesota, USA).