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Anti human car h 300

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The Anti-human CAR (H-300) is a laboratory reagent used for the detection and study of the human Chimeric Antigen Receptor (CAR) protein. It is a polyclonal antibody raised against a 300 amino acid epitope of the human CAR sequence.

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Lab products found in correlation

B220 apc ra3 6b2, by BD (2 mentions) Jaml pe, by BioLegend (2 mentions) Mouse alexa488 and rabbit alexa555, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho craf and phospho p44 42 mapk erk, by Cell Signaling Technology (2 mentions) Hrp conjugated anti gst, by GE Healthcare (2 mentions) Murine anti his, by Abcam (2 mentions) Cnbr sepharose 4 fast flow beads, by GE Healthcare (2 mentions) Ez link sulfo nhs lc biotin, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Hrp conjugated streptavidin, by Zymo Research (2 mentions)

Spelling variants of this product

H-300 (126 citations) Anti-CAR (5 citations)

2 protocols using anti human car h 300

1

Neutrophil and γδT Cell Activation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RmcB and 9E10 were purified from hybridomas. Anti-CD11b/CD18 (CBRM1/29) was previously described46 (link). Anti-human CAR (H-300) from Santa Cruz (Santa Cruz, CA). Murine PMN and γδT cells: CD3εeFluor450 (eBio500A2), CD45-PerCP-Cy5.5 (RM4-5), TCRγδ-FITC (GL3), Gr1-APC-eFluor780 (RB6-8C5), B220-APC (RA3-6B2) from BD Biosciences, and JAML-PE (4E10; BioLegend). Mouse-Alexa488 and rabbit-Alexa555 (Invitrogen, Carlsbad, CA), rabbit anti-Phospho-cRaf and Phospho-p44/42 MAPK (Erk ½) (Cell Signaling, Danvers, MA), mouse-HRP IgG, goat anti-rabbit Igγ (Jackson Immunoresearch, West Grove, PA). HRP-conjugated anti-GST (GE Healthcare), murine anti-His (Abcam, Cambridge, MA) and HRP-conjugated Streptavidin (Zymed, San Francisco, CA). Antibodies were biotinylated using Ezlink Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) or conjugated to CNBr-Sepharose 4 Fast Flow beads (GE Healthcare) according to manufacturers instructions. ABTS, (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), Phenanthroline monohydrate, N-Ethylmaleimide, Phenylmethylsulfonyl fluoride (PMSF), protease inhibitor cocktail (#P8340), PMSF, Phorbol Myristate Acetate (PMA), HBSS with/without Ca2+ and Mg2+ (H+/H-), fMLF and phosphatase inhibitors cocktails 1 and 2, Sigma (St Louis, MO). TNFα protease inhibitor-2 (TAPI-2) Calbiochem (Merk, Darmstadt, Germany).
Weber D.A., Sumagin R., McCall I.C., Leoni G., Neumann P.A., Andargachew R., Brazil J.C., Medina-Contreras O., Denning T.L., Nusrat A, & Parkos C.A. (2014). Neutrophil-derived JAML Inhibits Repair of Intestinal Epithelial Injury During Acute Inflammation. Mucosal immunology, 7(5), 1221-1232.
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2

Neutrophil and γδT Cell Activation Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RmcB and 9E10 were purified from hybridomas. Anti-CD11b/CD18 (CBRM1/29) was previously described46 (link). Anti-human CAR (H-300) from Santa Cruz (Santa Cruz, CA). Murine PMN and γδT cells: CD3εeFluor450 (eBio500A2), CD45-PerCP-Cy5.5 (RM4-5), TCRγδ-FITC (GL3), Gr1-APC-eFluor780 (RB6-8C5), B220-APC (RA3-6B2) from BD Biosciences, and JAML-PE (4E10; BioLegend). Mouse-Alexa488 and rabbit-Alexa555 (Invitrogen, Carlsbad, CA), rabbit anti-Phospho-cRaf and Phospho-p44/42 MAPK (Erk ½) (Cell Signaling, Danvers, MA), mouse-HRP IgG, goat anti-rabbit Igγ (Jackson Immunoresearch, West Grove, PA). HRP-conjugated anti-GST (GE Healthcare), murine anti-His (Abcam, Cambridge, MA) and HRP-conjugated Streptavidin (Zymed, San Francisco, CA). Antibodies were biotinylated using Ezlink Sulfo-NHS-LC-Biotin (Thermo Scientific, Rockford, IL) or conjugated to CNBr-Sepharose 4 Fast Flow beads (GE Healthcare) according to manufacturers instructions. ABTS, (2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), Phenanthroline monohydrate, N-Ethylmaleimide, Phenylmethylsulfonyl fluoride (PMSF), protease inhibitor cocktail (#P8340), PMSF, Phorbol Myristate Acetate (PMA), HBSS with/without Ca2+ and Mg2+ (H+/H-), fMLF and phosphatase inhibitors cocktails 1 and 2, Sigma (St Louis, MO). TNFα protease inhibitor-2 (TAPI-2) Calbiochem (Merk, Darmstadt, Germany).
Weber D.A., Sumagin R., McCall I.C., Leoni G., Neumann P.A., Andargachew R., Brazil J.C., Medina-Contreras O., Denning T.L., Nusrat A, & Parkos C.A. (2014). Neutrophil-derived JAML Inhibits Repair of Intestinal Epithelial Injury During Acute Inflammation. Mucosal immunology, 7(5), 1221-1232.
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