HepG2/C3A (RRID:CVCL_1098), SK-HEP-1 (RRID:CVCL_0525), Hepa1-6 (RRID:CVCL_0327), and HEK293T (RRID:CVCL_0063) cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Huh7 cells (RRID:CVCL_2957) purchased from Sigma-Aldrich. Huh7, HepG2/C3A, SK-HEP-1, Hepa1-6, and HEK293T cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS; Life Technologies, Thermo Fisher Scientific) and 1% penicillin/streptomycin (Life Technologies) in 5% CO2 at 37°C. All provided cell lines were obtained with confirmed STR profiling status by ATCC and Sigma-Aldrich. Mycoplasma negative status of all cell lines was ensured using universal mycoplasma detection kit from ATCC (30-1012K).
Hepg2 c3a
Manufactured by Merck Group
Sourced in United States
HepG2/C3A is a human hepatocellular carcinoma cell line derived from a liver biopsy. It is a well-characterized in vitro model used for various applications in cell biology and drug discovery research.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t, by Merck Group (1 mentions)
Hepg2 c3a, by American Type Culture Collection (1 mentions)
Sk hep 1, by Merck Group (1 mentions)
Hepa1 6, by Merck Group (1 mentions)
Huh 7 cells, by Merck Group (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Sk hep1, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Hepa1 6, by American Type Culture Collection (1 mentions)
Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Non essential amino acids (neaa), by Merck Group (1 mentions)
Plc prf 5, by Merck Group (1 mentions)
2 protocols using hepg2 c3a
1
Cell Line Procurement and Maintenance
2
Culturing HepG2/C3A and PLC/PRF/5 HCC Cells
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HepG2/C3A and PLC/PRF/5 HCC cells were purchased from ATCC (American Type Cell Culture, USA) and maintained as recommended. HepG2/C3A cells were cultured in DMEM (1g/L glucose) containing 10% fetal bovine serum, 1% penicillin/streptomycin (Sigma Chemical Co., St. Louis, MO, USA) and 1% non-essential amino acids (NEAA, Sigma Chemical Co., St. Louis, MO, USA). PLC/PRF/5 cells were cultured in DMEM (1g/L glucose) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Sigma Chemical Co., St. Louis, MO, USA) but no NEAA. Both cell lines were incubated at 37°C in a humid incubator with 5% CO2.
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