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7 protocols using ferric chloride fecl3 6h2o

1

Synthesis of Iron Oxide Nanoparticles

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All the reagents used in this work including ferrous chloride FeCl2.4H2O (M.W. 198.81), ferric chloride FeCl3.6H2O, and sodium hydroxide (NaOH) were of analytic grade and purchased from Sigma Aldrich, UK.
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2

Antioxidant Capacity Assays of Chemicals

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Methanol was obtained from Sigma-Aldrich (St. Louis, MO, USA). The Folin–Ciocalteu reagent was supplied by Panreac (Barcelona, Spain). Sodium carbonate and trichloroacetic acid (TCA) were obtained from Fluka (Steinheim, Switzerland). Gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, and ferric chloride (FeCl3·6H2O) were supplied by Sigma-Aldrich. Phosphate buffer (pH 6.6) was prepared from sodium dihydrogen phosphate (NaH2PO4·2H2O; Merck, Darmstadt, Germany) and disodium hydrogen phosphate (Na2HPO4·2H2O; Panreac).
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3

Synthesis of Polyaniline Nanocomposites

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All chemicals were of analytical grade. Polyvinyl alcohol with a degree of hydrolysis of 89% (molecular weight, Mw, of about 115.000 g mol−1, Sigma-Aldrich), ferrous chloride (FeCl2·4H2O (Sigma-Aldrich)), ferric chloride (FeCl3·6H2O) (Sigma-Aldrich), sodium hydroxide (NaOH) (Sigma-Aldrich), deionized water, aniline (Sigma-Aldrich), dodecylbenzene sulfonic acid (DBSA), and ammonium persulfate ((NH4)2S2O8) (Sigma-Aldrich) were used without further purification.
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4

Analytical-grade Reagent Synthesis

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Analytical-grade K2Cr2O7 (≥ 99.0%), Orange II sodium salt (AO7 dye) (dye content ≥ 85 %), ferrous chloride (FeCl2.4H2O) (≥ 99.0%), sodium hydroxide (NaOH) (≥ 98%), and ferric chloride (FeCl3·6H2O) (97%) were purchased from Sigma-Aldrich. All the solutions were prepared in distilled water.
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5

Synthesis and Characterization of Chitosan-Iron Composites

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All chemicals used were of analytical reagent grade and were used without further purification. CS (low molecular weight, deacetylation 75%–85%) was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Ferric chloride (FeCl3·6H2O) with 99% purity and ferrous chloride (FeCl2·4H2O) (>99% purity) were obtained from Merck KGaA (Darmstadt, Germany), sodium hydroxide (28% purity), and Strep sulfate were obtained from Sigma-Aldrich. An acetic acid solution (99.8% purity) was purchased from Hamburg Industries Inc (Hamburg, Germany). All aqueous solutions were prepared using deionized water.
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6

Diabetic Oxidative Stress Biomarkers

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Coenzyme Q10 was obtained from Cayman medical company (Ann Arbor, MI, USA) and streptozotocin (STZ) was obtained from sigma (St. Louis, MO, USA). RNX-plus reagent was purchased from Sinaclon company (Tehran, I.R. Iran) and reagents for complementary DNA (cDNA) synthesis were acquired from Takara (Kyoto, Japan). RT-PCR SYBR green I master mix was obtained from Yekta Tajhiz Azma Co. (Tehran, I.R. Iran). SOD kit was procured from Zell bio GmbH Company (Germany). 2,4,6-Tripyridyl-s-triazine, 1,1,3,3-tetraethoxy propane, were obtained from Sigma (St. Louis, MO, USA). 2-thiobarbituric acid, ferric chloride (FeCl3, 6H2O), and sodium acetate were provided by Merck company (Darmstadt, Germany).
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7

Microwave-Assisted Synthesis of Iron Oxide Nanoparticles from Walnut Shell

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The walnut shell (WS) was collected from the southwestern of Morocco (Taroudant city). The walnut shell (WS) was washed with tap water and distilled water. The washed walnut shell (WS) was oven dried at 60 °C for 12 h. The iron precursor was ferric chloride (FeCl3·6H2O) and was purchased from Merck Millipore. Salicylic acid (SA), naproxen (Nap), and ketoprofen (Keto) were acquired from Sigma-Aldrich. Moreover, pH values of the solutions were controlled with HCl (Merck Millipore) and NaOH (Merck Millipore) solutions. All chemicals were used as received and all solutions were prepared with distilled water. The synthesis was performed in a Teflon autoclave using microwave radiation (Delonghi EMD MW 311 adjustable power < 800 W, 230 V, 50 Hz).
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