Prochlorperazine dimaleate

The human glioblastoma cells U-87 MG were obtained from the Sigma Aldrich (USA)-European Collection of Authenticated Cell Cultures (ECACC) 89081402. The U-87 MG cell line was authenticated by ECACC by STR profiling with the use of PowerPlex 16 HS PCR amplification kit. Glioblastoma cells were cultured in Dulbecco's modified Eagle medium (DMEM), constituting a basal medium, which was supplemented with fetal bovine serum (FBS) (10%), neomycin (10 µg/ml), amphotericin B (0.25 µg/ml), and penicillin G (100 U/ml) at 37°C in 5% CO₂. Perphenazine, prochlorperazine dimaleate, bacitracin, elacridar, dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), amphotericin B, and penicillin G were purchased from Sigma-Aldrich Inc. (USA). Neomycin sulfate was obtained from Amara (Poland). Trypsin/EDTA 0.25/0.02% in PBS, FBS EU professional heat-inactivated and growth medium DMEM with 4.5 g/l Glucose, L-glutamine, and 3.7 g/l NaHCO₃ were obtained from PAN Biotech GmbH (Germany). Geltrex LDEV-Free reduced growth factor basement membrane matrix without Phenol Red was obtained from Gibco (USA). Methanol, acetic acid, and crystal violet were obtained from POCH S.A. (Poland). Buffered formalin was obtained from Chempur (Poland).