Synergy neo2 plate

Manufactured by Agilent Technologies

The Synergy Neo2 is a multi-mode microplate reader designed for a variety of applications in life science research. It features high-performance detection capabilities, including absorbance, fluorescence, and luminescence measurements, to support a wide range of assay types. The device is intended to provide accurate and reliable data for researchers in various fields.

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Lab products found in correlation

Flat bottom 96 well microtiter plates, by Corning (2 mentions) Apotox glo assay, by Promega (1 mentions) 384 well plate, by Corning (1 mentions) Blocker casein, by Thermo Fisher Scientific (1 mentions) Tmb microwell peroxidase, by LGC (1 mentions) Goat anti human igg fc secondary antibody with hrp, by Thermo Fisher Scientific (1 mentions) Maxisorp plate, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Synergy Neo2 Hybrid Multi-Mode Plate Reader Biotek synergy NEO2 fluorescent plate reader Synergy Neo2 Multi-Mode Plate Reader Synergy Neo2 plate reader Synergy™ Neo2 HTS Multi-Mode Plate Reader Synergy Neo2

5 protocols using synergy neo2 plate

Bioluminescence Assay for Bacterial Growth

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Overnight cultures were diluted to OD₆₀₀ ~0.05 and grown in TSB at 37ºC with rotary shaking at 180 rpm. Aliquots (100 μL) were inoculated into flat bottom 96-well microtiter plates (Corning, Corning, NY), and bioluminescence was detected using a BioTek Synergy Neo2 plate reader (Agilent, Santa Clara, CA).

Podkowik M., Perault A.I., Putzel G., Pountain A., Kim J., Dumont A., Zwack E., Ulrich R.J., Karagounis T.K., Zhou C., Haag A.F., Shenderovich J., Wasserman G.A., Kwon J., Chen J., Richardson A.R., Weiser J.N., Nowosad C.R., Lun D.S., Parker D., Pironti A., Zhao X., Drlica K., Yanai I., Torres V.J, & Shopsin B. (2023). Quorum-sensing agr system of Staphylococcus aureus primes gene expression for protection from lethal oxidative stress. bioRxiv.

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Bacterial Growth Kinetics Monitoring

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Overnight cultures were diluted to OD₆₀₀ ~0.05 and grown in TSB at 37 °C with rotary shaking at 180 rpm. Aliquots (100 μL) were inoculated into flat bottom 96-well microtiter plates (Corning, Corning, NY), and bioluminescence was detected using a BioTek Synergy Neo2 plate reader (Agilent, Santa Clara, CA).

Podkowik M., Perault A.I., Putzel G., Pountain A., Kim J., DuMont A.L., Zwack E.E., Ulrich R.J., Karagounis T.K., Zhou C., Haag A.F., Shenderovich J., Wasserman G.A., Kwon J., Chen J., Richardson A.R., Weiser J.N., Nowosad C.R., Lun D.S., Parker D., Pironti A., Zhao X., Drlica K., Yanai I., Torres V.J, & Shopsin B. (2024). Quorum-sensing agr system of Staphylococcus aureus primes gene expression for protection from lethal oxidative stress. eLife, 12, RP89098.

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Temporal Analysis of miR-124 Depletion

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WT and ΔmiR-124 were seeded and differentiated consecutively according to the time frame from 0 to 14 dpi and all samples were measured with a SynergyNeo2 plate reader (BioTek) on the same day. The ApoToxGlo assay (G6320, Promega) was conducted according to the manufacturer’s protocol. Statistical comparison was performed using unpaired Student’s t-tests and Holm-Sidak correction for multiple comparisons. Data are presented as mean ± SEM.

Kutsche L.K., Gysi D.M., Fallmann J., Lenk K., Petri R., Swiersy A., Klapper S.D., Pircs K., Khattak S., Stadler P.F., Jakobsson J., Nowick K, & Busskamp V. (2018). Combined Experimental and System-Level Analyses Reveal the Complex Regulatory Network of miR-124 during Human Neurogenesis. Cell Systems, 7(4), 438-452.e8.

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Spectrophotometric Assay of Proteasome ATPase Activity

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Proteasome ATPase activity was monitored using a spectrophotometric assay that couples regeneration of hydrolyzed ATP to the oxidation of NADH (51 (link)). Reactions contained a final concentration of 150 nM 26S proteasome that had been preincubated with ortho-phenanthroline and ATPase mix for 5 min on ice or mock treated before bringing the sample to 25°C and adding FAM-labeled ubiquitinated substrate to a final concentration of 3 µMand ortho-phenanthroline to a final concentration of 3 mM. Absorbance at 340 nm was measured for 10 min with 12-s intervals in a 384-well plate (Corning) using a Biotek Synergy Neo2 plate reader. Reactions were done in 60 mM HEPES, pH 7.6, 20 mM NaCl, 20 mM KCl, 10 mM MgCl₂, 2.5% glycerol 1 mM TCEP, and 1 X ATPase mix (5 mM ATP, 3 U ml⁻¹ pyruvate kinase, 3 U ml⁻¹ lactate dehydrogenase,1 mM NADH, and 7.5 mM phosphoenol pyruvate).

de la Peña A.H., Goodall E.A., Gates S.N., Lander G.C, & Martin A. (2018). Substrate-engaged 26S proteasome structures reveal mechanisms for ATP-hydrolysis–driven translocation. Science (New York, N.Y.), 362(6418), eaav0725.

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ELISA for Serological Detection of Henipavirus Glycoproteins

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96-well Maxisorp plates (Thermo Fisher) were coated overnight at 4°C with 2 μg/mL of NiV G or LayV G central stalk stabilized ectodomain in 50mM Tris and 150mM NaCl at pH 8. Plates were slapped dry, washed 3X in Tris Buffered Saline Tween (TBST) and blocked with Blocker Casein (ThermoFisher) for 1 h at 37°C. Plates were slapped dry and washed 4X in TBST. 1:4 serial dilutions of NHP sera were made in 50 μL TBST and incubated at 37°C for 1 h. Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C. Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL TMB Microwell Peroxidase (Seracare). The reaction was quenched after 4 minutes with 1 N HCl and the A450 of each well was read using a BioTek Synergy Neo2 plate reader. Data was plotted and fit in Prism (GraphPad) using nonlinear regression sigmoidal, 4PL, X is log(concentration).

Wang Z., McCallum M., Yan L., Sharkey W., Park Y.J., Dang H.V., Amaya M., Person A., Broder C.C, & Veesler D. (2023). Structure and design of Langya virus glycoprotein antigens. bioRxiv.

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