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Mini trek

Manufactured by Abbott
Sourced in United States

The MINI TREK is a compact and portable laboratory equipment designed for molecular biology applications. It provides a reliable platform for performing basic DNA/RNA amplification techniques such as PCR. The MINI TREK offers consistent temperature control and intuitive operation, making it a versatile tool for various research and clinical settings.

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3 protocols using mini trek

1

Ethanol Ablation of the Vein of Marshall

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The coronary sinus (CS) was cannulated with a steerable long sheath (Agilis NxT; Abbott, St Paul, MN) inserted from the right femoral vein. An angiography catheter (5F left internal mammary artery; Medtronic, Minneapolis, MN) was proximally positioned inside the CS lumen, and contrast (iodine) was injected to localize the VOM ostium. When not identified, a CS venogram was performed by dedicated balloon occlusion. Subsequently, an angioplasty wire (Whisper 0.014, Abbott or Sion Blue 0.014, Asahi) was advanced inside the VOM lumen and used to position a preloaded over-the-wire balloon (MINI TREK, 1.5–3 mm diameter and 6–15 mm length, Abbott) within the first 15 mm of its proximal portion. After inflation at 2 to 6 atm (until some resistance was felt) and wire removal, a selective angiography was performed through the wire port to confirm balloon occlusion and visualize VOM arborization. Absolute ethanol (96%) was collected in a metal bowel. Three successive injections (1–3 mL) were slowly administered over 1 minute, with selective VOM angiogram repeated each time to confirm balloon stability (Figure 1).7 (link)
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2

Rg2 Attenuates Carotid Injury in Rats

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Male Sprague-Dawley rats (280 g) were randomly divided into four groups (n = 5 per group): control group, injury group, and two Rg2 treatment groups (low and high dose). The two Rg2 groups were treated with 8 mg/kg/d and 40 mg/kg/d Rg2 for 14 d. First, a carotid balloon injury model in rats was constructed using a balloon catheter (Mini TREK, Abbott Laboratories, Chicago, IL, USA) inserted into the common carotid artery through the left external carotid artery, and was swollen five times with 0.9% saline. The common carotid artery was ligated after pulling out the catheter. Post-surgery, rats in all the groups were administered carboxyl methyl cellulose sodium (CMC-Na) and Rg2 by gavage for two weeks. The day after the last administration, an intraperitoneal injection of 10% chloral hydrate was administered and the blood vessels of the left common artery were extracted for morphological examination and subsequent experiments–frozen section staining, Western blot analysis, and total RNA.
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3

Ethanol Ablation of Vein of Marshall

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VOM-Et was performed as the first step in all patients, using a retrograde approach via the femoral vein. 14 (link) A long steerable sheath (Agilis NxT, Abbott, MN) was advanced into the CS for stability during insertion of a 5-French left internal mammary artery angiography catheter (Medtronic, Minneapolis, MN). After selective venography of the VOM, an angioplasty wire (whisper 0.014 inches, Abbott or Sion Blue 0.014, Asahi) and an over-the-wire balloon catheter (1.5-2.5 mm diameter and 6-15 mm length, 145 cm MINI TREK, Abbot) were advanced. Subsequently, the balloon was inflated at a low pressure until the operator felt resistance (2-6 atm). After removing the guidewire, 3 successive injections of ethanol 96% ethanol were performed, with VOM venography repeated after each injection to confirm stability of the balloon and absence of a leakage into the CS (Figure 1). Thereafter, additional CS-ABL was systematically performed with the support of the steerable sheath, from the level of the left inferior PV to the ostium of the VOM. Subsequent PV isolation, LA roof ablation, MI ablation, and cavotricuspid isthmus ablation were performed. The endocardial MI line was located posteriorly through the low-voltage area created by VOM-Et, at areas still capable of conduction.
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