Mononuclear cells were isolated from peripheral blood using ficoll density gradient centrifugation and stained with the following anti-human antibody staining reagents: Goat F(ab')2 Anti-Human IgD- FITC (Invitrogen) or -APC (clone: IgD26, Miltenyi Biotec); Goat F(ab')2 Anti-Human IgM-PE-Cy5; CD3-Pacific Orange (clone: UCHT1), CD14-Pacific Orange (clone: TK4); CD24-PE-A610 (clone: SN3; Invitrogen); CD19-APC-Cy7 (clone: SJ25C1); CD38-Pacific Blue (clone: HB7); CD23-PE-Cy7 clone: M-L233); CD21-PE-Cy5 (clone B-ly4); CD27-PE (clone: L128, BD Pharmingen); CD138-APC (clone: 44F9, Miltenyi Biotec) and mitotracker green. Approximately 0.1–3 × 105 cells were collected for each population using a BD FACS Aria II (BD Biosciences).
Cd38 pacific blue
Manufactured by BD
Sourced in United States
CD38-Pacific Blue is a fluorochrome-conjugated antibody used for the detection and analysis of CD38 expression on cells by flow cytometry. CD38 is a transmembrane glycoprotein that functions as an ectoenzyme involved in the regulation of cell adhesion, activation, and signaling. The Pacific Blue fluorochrome is used to label the CD38 antibody, allowing for its identification and quantification on cell surfaces.
Automatically generated - may contain errors
Lab products found in correlation
Cd21 pe cy5, by BD (2 mentions)
Cd138 apc, by Miltenyi Biotec (2 mentions)
Cd3 pacific orange, by Thermo Fisher Scientific (2 mentions)
Goat f ab 2 anti human igd fitc, by Thermo Fisher Scientific (2 mentions)
Goat f ab 2 anti human igm pe cy, by Thermo Fisher Scientific (2 mentions)
Cd14 pacific orange, by Thermo Fisher Scientific (2 mentions)
Cd24 pe a610, by Thermo Fisher Scientific (2 mentions)
Cd19 apc cy7, by Thermo Fisher Scientific (2 mentions)
Cd23 pe cy7, by BD (2 mentions)
Cd27 pe, by BD (2 mentions)
Facsaria 2, by BD (2 mentions)
3 protocols using cd38 pacific blue
1
Flow Cytometric Analysis of Human B Cell Subsets
2
Flow Cytometric Analysis of Human B Cell Subsets
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Mononuclear cells were isolated from peripheral blood using ficoll density gradient centrifugation and stained with the following anti-human antibody staining reagents: Goat F(ab')2 Anti-Human IgD- FITC (Invitrogen) or -APC (clone: IgD26, Miltenyi Biotec); Goat F(ab')2 Anti-Human IgM-PE-Cy5; CD3-Pacific Orange (clone: UCHT1), CD14-Pacific Orange (clone: TK4); CD24-PE-A610 (clone: SN3; Invitrogen); CD19-APC-Cy7 (clone: SJ25C1); CD38-Pacific Blue (clone: HB7); CD23-PE-Cy7 clone: M-L233); CD21-PE-Cy5 (clone B-ly4); CD27-PE (clone: L128, BD Pharmingen); CD138-APC (clone: 44F9, Miltenyi Biotec) and mitotracker green. Approximately 0.1–3 × 105 cells were collected for each population using a BD FACS Aria II (BD Biosciences).
3
CXCL4-Nucleic Acid Complexes Modulate B-Cell Maturation
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CXCL4 was premixed with DNA (10 µg/mL) or RNA (15 µg/mL) and added to the B-cell cultures after a 15-min incubation at room temperature. The concentration of CXCL4 was between 1–2 µM. Complexes were formed with huDNA/BacDNA, or huRNA/BacRNA. In some experiments, B-cells were stimulated with CXCL4 (1 µM) in complex with low molecular weight heparin (calcium nadroparin, 5 UI/mL). Moreover, cells were treated with CXCL4–DNA complexes, followed by treatment, after 30 min with heparin at 5 UI/mL; alternatively, cells were treated with CXCL4 (1 µM) plus heparin (5 UI/mL) and, after 30 min, DNA (10 µg/mL) was added to the cultures.
Maturation of B-cells into plasma cells was investigated by flow cytometry (Gallios, Beckman Coulter, Brea, CA, USA) with monoclonal anti-human CD19 fluorescein, CD27 allophycocyanin, CD38 Pacific Blue, CD138 phycoerythrin (BD Biosciences, CA, USA) antibodies at day 7.
Maturation of B-cells into plasma cells was investigated by flow cytometry (Gallios, Beckman Coulter, Brea, CA, USA) with monoclonal anti-human CD19 fluorescein, CD27 allophycocyanin, CD38 Pacific Blue, CD138 phycoerythrin (BD Biosciences, CA, USA) antibodies at day 7.
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