Copper 2 sulfate pentahydrate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Copper (II) sulfate pentahydrate is a chemical compound with the formula CuSO4·5H2O. It is a blue crystalline solid that is widely used in various industries and applications. The compound consists of copper, sulfur, and water molecules. Copper (II) sulfate pentahydrate is a versatile chemical that can be used in a variety of laboratory applications.

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Copper sulfate (9 citations) Copper sulfate pentahydrate (7 citations) Copper (II) sulfate (7 citations) Copper(II) sulfate pentahydrate (CuSO4·5H2O, (4 citations)

5 protocols using copper 2 sulfate pentahydrate

Synthesis and Characterization of Click-Functionalized Peptides

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We purchased HA sodium salt (molecular weight 16 kDa) from Lifecore Biomedical; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide from TCI Chemicals; 2-(N-morpholino)ethane-sulfonic acid sodium salt, tris(3-hydroxypropyltriazolylmethyl)amine, and sodium ascorbate from Sigma-Aldrich; and 11-azido-3,6,9-trioxaundecan-1-amine, N-hydroxysuccinimide, and copper (II) sulfate pentahydrate from Acros Organics. Alkyne-functionalized peptides bearing an N-terminal 4-pentynoic acid (homopropargyl) modification, hpP79 and aoP79, were obtained from Biomatik. Additionally, alkyne-functionalized peptides bearing an N-terminal alkyne polyethylene glycol group modification, 2.5HIP and a control p79 with two permuted amino acids inactive on BDC2.5 T cells, were purchased from PolyPeptide. All reagents were used as received without further purification.

Firdessa-Fite R., Johnson S.N., Leon M.A., Khosravi-Maharlooei M., Baker R.L., Sestak J.O., Berkland C, & Creusot R.J. (2021). Soluble Antigen Arrays Efficiently Deliver Peptides and Arrest Spontaneous Autoimmune Diabetes. Diabetes, 70(6), 1334-1346.

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Copper-tolerant Bacterial Strains Isolation

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All reagents were obtained from commercial vendors and used without further purification. These include: tetraethyl orthosilicate (TEOS, 98%, Gelest Inc., PA, USA), didecyldimethylammonium chloride (DDAC, 50% solution in 2-propanol/water 2 : 3 from EMD Millipore, MA, USA), ethanol (190 proof, Pharmco, CT, USA), ethanol (200 proof, Acros Organics, NJ, USA) and copper(ii) sulfate pentahydrate (99+%) (Acros Organics, NJ, USA), nitric acid (68 to 70% (w/w), Fisher Scientific, PA, USA), sodium hydroxide solid beads (Fisher Scientific, PA, USA), nutrient broth (NB) and agar (Fluka, St. Louis, MO, USA). Xanthomonas alfalfae subsp. citrumelonis (ATCC 49120), Pseudomonas syringae pv. syringae (ATCC 19310), and Clavibacter michiganensis subsp. michiganensis (ATCC 10202) cultures from ATCC (U.S. Department of Agriculture (USDA) permits P526P-12-04060 and P526P-15-01601) and X. perforans strains GEV485 (Cu-tolerant) and 91-118 (Cu-sensitive) isolated from tomato in Florida provided by Paret and Jones (North Florida Research and Education Center, University of Florida, USA) were used. Kocide 3000® (DuPont™) is a Cu-based fungicide/bactericide retaining 46.1% copper hydroxide (CAS No. 20427-59-2) available for commercial use for growers.

Ozcan A., Young M., Lee B., Liao Y.Y., Da Silva S., Godden D., Colee J., Huang Z., Mendis H.C., Campos M.G., Jones J.B., Freeman J.H., Paret M.L., Tetard L, & Santra S. (2021). Copper-fixed quat: a hybrid nanoparticle for application as a locally systemic pesticide (LSP) to manage bacterial spot disease of tomato. Nanoscale Advances, 3(5), 1473-1483.

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Synthesis of Copper Nanoparticles

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Absolute ethanol (C₂H₅OH, purity ≥ 99.8%), copper (II) sulfate pentahydrate (CuSO₄·5H₂O, purity ≥ 98.0%), ascorbic acid (C₆H₈O₆, purity ≥ 99.7%), and ammonia hydroxide (NH₃·H₂O, 35%) were purchased from Fisher Chemical (Pittsburgh, PA, USA) and used without further purification. Polyvinylpyrrolidone (PVP, purity ≥ 99.0%), ethylene glycol (C₂H₄(OH)₂, purity ≥ 99.5%), formic acid (CH₂O₂, purity ≥ 95.0%), and sodium perchlorate (NaClO₄, purity ≥ 99.0%) were obtained from Acros Organics (Geel, Belgium). Commercial wetting agent (type 2230) was used in this study, supplied by Marvel Chemical Co. (Taipei, Taiwan). PET ((C₁₀H₈O₄)_n) was purchased from Hsin Yun Co. (Taipei, Taiwan). All chemicals were analytical grade and used as received.

Hong G.B., Wang J.F., Chuang K.J., Cheng H.Y., Chang K.C, & Ma C.M. (2022). Preparing Copper Nanoparticles and Flexible Copper Conductive Sheets. Nanomaterials, 12(3), 360.

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Fabrication of Zn-Cu Alloy Electrodeposits

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In total, 100 mL DI water was pre-heated as the solvent to dissolve zinc sulfate heptahydrate (ZnSO₄·7H₂O, Fisher Chemical), copper (II) sulfate pentahydrate (Fisher Chemical), and boric acid (Powder/Certified ACS, Fisher Chemical) under continuous stirring for 20 min until a transparent solution was obtained (noted as Solution C). The Zn-Cu alloys were deposited on Zn substrates using the two-electrode setup in Solution C.

Tian H., Li Z., Feng G., Yang Z., Fox D., Wang M., Zhou H., Zhai L., Kushima A., Du Y., Feng Z., Shan X, & Yang Y. (2021). Stable, high-performance, dendrite-free, seawater-based aqueous batteries. Nature Communications, 12, 237.

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Synthesis of Metal-Organic Frameworks

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Cobalt chloride
hexahydrate (CoCl₂·6H₂O, 98–102%)
and copper (II) sulfate pentahydrate (Cu(II)SO₄·5H₂O, 99%) were purchased from Fisher Scientific
(Ireland). All other reagents were purchased from Sigma Aldrich at
≥99% purity, apart from iron chloride hexahydrate (FeCl₃·6H₂O, 97%), zinc nitrate hexahydrate (Zn(NO₃)₂·6H₂O, 98%), sodium hydroxide
(NaOH >95%), trimesic acid (H₃BTC >95%), hexanal
(98%),
ethanol (96%), potassium phosphate dibasic (K₂HPO₄, ≥98%), hydrochloric acid (HCl, 37%), nitric acid (HNO₃, 70%), l-lactic dehydrogenase from rabbit muscle
(LDH, 800–1200 U/mg), lipase B Candida antarctica, recombinant
from Aspergillus oryzae (Lip, ∼9
U/mg), polyethylene glycol (PEG, average molecule weight 3400), PAA
(average M.W. ca. 2000), and polyethylenimine (PEI) (low molecular
weight), which were purchased from Sigma Aldrich (Ireland). All reagents
were used as received without further purification. Deionized water
(18.2 MΩ cm) was used for the preparation of all aqueous solutions.

Shortall K., Otero F., Bendl S., Soulimane T, & Magner E. (2022). Enzyme Immobilization on Metal Organic Frameworks: the Effect of Buffer on the Stability of the Support. Langmuir, 38(44), 13382-13391.

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