Western blot analysis was performed as a standard protocol. Exosomes, cells, or dorsal skin tissue were lysed by RIPA on ice, and then the protein concentration was measured by a BCA Protein Assay Kit (Thermo Fisher Scientific, USA). The primary antibodies were as follows: anti-phospho-GSK-3β (Ser9) (1 : 7,000, Rabbit monoclonal), anti-GSK-3β (1 : 7,000, Rabbit monoclonal), anti-β-catenin (1 : 7,000, Rabbit monoclonal), anti-Versican (1 : 2,000, Rabbit monoclonal), anti-ALP (1 : 1,000, Rabbit monoclonal), anti-GAPDH (1 : 10,000, Rabbit monoclonal), anti-CD9 (1 : 500, Rabbit monoclonal), anti-CD81 (1 : 500, Rabbit monoclonal), anti-TSG101 (1 : 2,000, Rabbit monoclonal), and anti-calnexin (1 : 3,000, Rabbit monoclonal) (Abcam, USA).
Anti phospho gsk 3β ser9
Manufactured by Abcam
Sourced in United States
Anti-phospho-GSK-3β (Ser9) is a primary antibody that recognizes the phosphorylated form of glycogen synthase kinase-3 beta (GSK-3β) at serine 9. GSK-3β is a key regulator of various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Anti gsk3β, by Abcam (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Anti versican, by Abcam (1 mentions)
Anti alp, by Abcam (1 mentions)
Anti calnexin, by Abcam (1 mentions)
Anti tsg101, by Abcam (1 mentions)
Anti cd81, by Abcam (1 mentions)
Anti cd9, by Abcam (1 mentions)
Anti phospho gsk 3β, by Abcam (1 mentions)
Anti ampkα, by Abcam (1 mentions)
Goat anti rabbit 7074, by Cell Signaling Technology (1 mentions)
Anti β actin, by Abcam (1 mentions)
Goat anti mouse 7076, by Cell Signaling Technology (1 mentions)
2 protocols using anti phospho gsk 3β ser9
1
Exosome and Tissue Protein Analysis
2
Western Blot Analysis of AMPK and GSK3β
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Protein collection and Western blot procedure were performed as described previously (11 (link)). Briefly, proteins were separated by SDS-polyacrylamide gels and transferred onto a PVDF membrane and incubated with the respective antibodies. The primary antibodies were diluted at 1:1,000, which include anti-AMPKα (#ab32047), anti-phospho-AMPKα Thr172 & Thr 183 (#ab23875), anti-phospho-GSK3β (#ab93926), anti-phospho-GSK3β Ser9 (#ab75814), anti-β-actin (#ab8226) antibodies and all the antibodies were purchased from Abcam (Cambridge, MA, USA). The secondary antibodies were diluted at 1:10000, including goat anti-rabbit (#7074) and goat anti-mouse (#7076) secondary antibodies purchased from Cell Signaling Technology (Beverly, MA, USA).
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