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PHDAC1 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a device designed for the analysis and detection of histone deacetylase activity. The core function of PHDAC1 is to measure the enzymatic activity of histone deacetylases in biological samples.

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2 protocols using phdac1

1

Molecular Techniques in Cancer Research

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The antibodies of Trib1, HDAC1, pHDAC1, p53, and shRNA kits of Trib1 and HDAC1 were from Santa Cruz Biotech (Shanghai, China). The Annexin V kit, protein G, butyrate sodium and Chip kit were from Sigma Aldrich (Shanghai, China). The reagents for RT-qPCR and Western blotting were from Invitrogen (Shanghai, China).
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2

Characterization of Regulatory T Cells

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The fluorochrome-labeled antibodies of IL-4, CD25, CD4, TGF-β and Foxp3 for flow cytometry were purchased from BD Biosciences (Beijing, China). The neutralizing antibody of MMP1a, ELISA kits of IL-4, IFN-γ, granzyme B, perforin and TGF-β were purchased from R&D Systems (Beijing, China). The Annexin V-Cy5 reagent kit, Imprint® Chromatin Immunoprecipitation (ChIP) Kit and protein G were purchased from Sigma Aldrich (Beijing, China). The antibodies of MMP1a, GATA3, Foxp3, HDAC1, pHDAC1, PAR2, CD9, CD63, CD81, MHC II and shRNA kit of HDAC1 were purchased from Santa Cruz Biotech (Beijing, China). The recombinant MMP1a was synthesized by GeneScript (Nanjing, China). The immune cell isolation kits were purchased from Miltenyi Biotech (Beijing, China).
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