Rabbit anti pikachurin

The following antibodies and concentrations were used: rabbit anti-Gγ13 (1:500, a gift from R. Margolskee); rabbit anti-Gβ3 (1:300, HPA005645; Sigma); guinea pig anti-mGluR6 (1:100, Neuromics Edina, MN Oregon); mouse anti-Gα_o (1:100, MAB 3073; Millipore); rabbit anti-TRPM1 (1:100, a gift from T. Furukawa, Osaka Bioscience Institute, Osaka, Japan) (Koike et al., 2010 (link); Kondo et al., 2011 (link)); rabbit anti-Gβ5 (1:500) and rabbit anti-RGS11 (1:1000, gifts from T. Wensel, Baylor College of Medicine, Houston, TX); rabbit anti-RGS7 (1:200, gift from K. Martemyanov, Scripps Research Institute, Jupiter, FL); rabbit anti-Ret-PCP2 (1:1,000, gift of Dr. Rod Feddersen; see Xu et al. 2008 (link)); rabbit anti-pikachurin (1:2,000, Wako 011-22631, Richmond,VA); mouse anti-dystroglycan (1:100, Abcam, ab49515, Cambridge, MA); mouse anti-dystrophin (1:100, Millipore MAB 1694, Billerica, MA); rabbit anti-RGS9 anchoring protein (R9AP) (1:100–500, a gift from V. Arshavsky, Duke University, Durham, NC); mouse anti-kinesin [1:100–200, Covance (currently Bio-legends)]; rabbit anti-ribeye (1:500, a gift from T. Sudhof, Stanford Universty, CA); sheep anti-TRPM1 (for Western blotting,1:1,000, Gift of Dr. Kirill Martemayanov, The Scripps Research Institute, Jupiter, Florida); and mouse anti-beta-actin (1:3,000, Clone AC-74 Sigma, St. Louis, MO).