Poly ethylene glycol methyl ether block poly lactide co glycolide

Phenyl alkoxyphenyl PPV copolymer conjugated polymers Livilux™ PDO-124 Super Orange (SO-PPV) and Livilux™ PDY-132 Super Yellow (SY-PPV) were supplied by Merck, Germany. Poly (9, 9-di-n-octyl-2,7-fluorene) (PFO), poly (9,9-dioctylfluorene-2,1,3-benzothiadiazole) (F8BT), poly (2-methoxy-5 -(2′-ethylhexyloxy)-1,4-phenylene vinylene) (MEH-PPV), poly (2.5-di (hexyloxy)cyanotere-phthalylidene) (CN-PPV), and the diblock copolymer poly (ethylene glycol) methyl ether-block-poly (lactide-co-glycolide) with 50:50 ratio of lactide/glycolide (PEG_5K-PLGA_55K), and tetrahydrofuran (THF ReagentPlus^®, ≥99.0%, catalog # 178810)) were supplied by Sigma-Aldrich Corporation (St. Louis, MO, United States).

The poly(lactide-co-glycolide) (PLGA) (Resomer^® RG 502H uncapped PLGA 50:50, inherent viscosity 0.16–0.24 dL/g) was acquired from Boehringer Ingelheim (Ingelheim, Germany). Poly(lactic-co-glycolic acid) copolymer-Rhodamine-B conjugate (Mn 10,000–30,000 Da) (PLGA-Rhod) was purchased from PolyScitech (Akina division, Inc., West Lafayette, IN, USA). Poly(ethylene glycol) methyl ether-block-poly(lactide-co-glycolide) with PEG average Mn 2000 Da, PLGA Mn 4500 Da and with PEG average Mn 5000 Da, PLGA Mn 7000 Da, diethylenetriaminepentaacetic acid (DTPA), DNA, egg yolk emulsion, poly(L-lysine), RPMI amino acid solution and type II mucin from porcine stomach were purchased from Merck-Sigma Aldrich (St. Louis, MO, USA). Bacterial alginate from the fermentation of Azotobacter vinelandii or Pseudomonas mendocina was purchased from Carbosynth (UK). Methylene chloride and ethanol were supplied by Carlo Erba (Italy). All salts and reagents were of analytical grade or higher.