Fibrin

Manufactured by Thermo Fisher Scientific

Sourced in China

Fibrin is a protein that plays a crucial role in the blood clotting process. It is formed from fibrinogen, a soluble plasma protein, through the action of the enzyme thrombin. Fibrin helps to create a mesh-like structure that traps blood cells and platelets, forming a clot to stop bleeding and promote healing.

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Lab products found in correlation

Cacl2, by Solarbio (1 mentions) Lipase, by Solarbio (1 mentions) Pcl mn 80 000, by Merck Group (1 mentions) Cell counting kit 8 (cck8), by Apexbio (1 mentions) Dulbecco s modified eagle medium dmem, by Dingguo (1 mentions)

3 protocols using fibrin

Fibrin-based Tissue Engineering Protocol

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PU was purchased from Sigma-Aldrich (Cat No.81367–5G, America). Fibrin was provided by Thermo Scientific company (Cat No.MA126074, America). Ninety-eight percent formic acid was provided by Aladdin company (Cat No.F112034, China). Dulbecco’s modified eagle’s medium (DMEM), phosphate buffer saline (PBS, Cat No.P1022-500), fetal bovine serum (FBS, Cat No.S9030-200), CaCl₂ (Cat No.G0071) and lipase (Cat No.L8621) were from Solarbio (Beijing, China). Living cell staining kit (Cat No.C2015S), CCK-8 kit (Cat No.C0038), ELISA kit (Cat No.PI328), KCL (Cat No.ST1596) were purchased from Beyotime. Sodium nitroprusside (SNP, Cat No.S817931), adrenaline (AD, Cat No.N814761) and acetylcholine (Ach, Cat No.A886059) were obtained from Macklin (Shanghai, China). Anti-von Willebrand Factor antibody (ab115771), Anti-α-SMA (251411), Mouse Anti-CD68 antibody were obtained from Ruizekang Biotechnology Co., Ltd. (Beijing, China). Safranin O (Cat No.G1031), Verhoeff (Cat No. G1046) and Sirius red (Cat No.G1038) kit were supplied by Servicebio Biochemical Technology Co., Ltd (Wuhan, China). Mesenchymal stromal cells (MSCs) were obtained from Xinxiang Medical University (Henan, China). The use of MSCs has been approved by the Ethics Committee of Xinxiang Medical University.

Yang L., Li X., Wu Y., Du P., Sun L., Yu Z., Song S., Yin J., Ma X., Jing C., Zhao J., Chen H., Dong Y., Zhang Q, & Zhao L. (2020). Preparation of PU/Fibrin Vascular Scaffold with Good Biomechanical Properties and Evaluation of Its Performance in vitro and in vivo. International Journal of Nanomedicine, 15, 8697-8715.

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Tissue Engineering Scaffold Fabrication

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PCL (Mn:80,000) was come from Sigma Aldrich Trading Co., Ltd. (Shanghai, China). Fibrin was acquired from Thermo Fisher. 98% formic acid, 5% fetal bovine serum, TritonX-100, monoclonal antibody secondary antibody and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Jisi Instrument Equipment Co., Ltd. (Wuhan, China). 10%KCl, adrenaline, Ach, SNP, 4% paraformaldehyde, Masson staining kit, hematoxylin eosin staining kit, Safranin O, Verhoeffi-Van Gieson staining (VVG) and von Kossa staining kit were obtained from Sevier Biotechnology Co., Ltd. (Wuhan, China). Rabbit anti-von Willebrand factor (vWF) antibody, mouse monoclonal anti-CD31, mouse anti-α-SMA, anti-CD34 and mouse anti-SM-MHC were come from the Solarbio Co., Ltd. (Beijing, China). Mouse inflammatory factor ELISA detection kit was bought from Jianglai Biotechnology Co., Ltd. (Shanghai, China).

Zhao L., Li X., Yang L., Sun L., Mu S., Zong H., Li Q., Wang F., Song S., Yang C., Zhao C., Chen H., Zhang R., Wang S., Dong Y, & Zhang Q. (2020). Evaluation of remodeling and regeneration of electrospun PCL/fibrin vascular grafts in vivo. Materials Science & Engineering. C, Materials for Biological Applications, 118, 111441.

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Silk Fibroin Scaffold for Tissue Engineering

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Fibrin was provided by Thermo Fisher (Wuhan, China). Bombyx mori silk cocoons were obtained from Chuangseed Biomaterials Company (Guangzhou, China). 98% formic acid and Dulbecco's Modified Eagle Medium (DMEM) was obtained from Dingguo Biotechnology Co., Ltd (Zhengzhou, China). Cell Counting kit-8, Masson staining kit, CD68, hematoxylin eosin staining kit and Live Cell Staining Kit were acquired from APExBIO Technology LLC (Shanghai, China). Fetal bovine serum, actin antibody, DAPI, Triton-X100, PBS and 4%polyformaldehyde solutions were purchased from Weigo Technology Co., Ltd (Guangzhou, China).

Yang L., Wang X., Xiong M., Liu X., Luo S., Luo J, & Wang Y. (2024). Electrospun silk fibroin/fibrin vascular scaffold with superior mechanical properties and biocompatibility for applications in tissue engineering. Scientific Reports, 14, 3942.

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