Human melanoma cell lines A375, RPMI, and SK-MEL-28; human keratinocytes; and HaCaT were purchased from the American Type Culture Collection (ATCC) and human skin fibroblast, 1BR3 was obtained from the European Collection of Authenticated Cell Cultures (ECACC). A375 and HaCaT were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich) high-glucose medium supplemented with 10% fetal bovine serum (FBS, Gibco, ThermoFisher Scientific Waltham, MA, USA) and 1% penicillin/Strep, 10,000 IU/mL, Sigma-Aldrich, St. Louis, MO, USA) and RPMI, SK-MEL-28 and 1BR3 were cultured in Eagle’s Minimum Essential Medium (EMEM, Sigma-Aldrich, St. Louis, MO, USA) high-glucose medium supplemented with 10% fetal bovine serum (FBS, Gibco, ThermoFisher Scientific, Waltham, MA, USA) and 1% penicillin/Strep, 10,000 IU/mL, Sigma-Aldrich, St. Louis, MO, USA). The cells were incubated under standard temperature conditions of 37 °C and humidity containing 5% CO2.
Penicillin strep
Manufactured by Merck Group
Sourced in United States
The Penicillin/Strep is a piece of lab equipment used to cultivate and isolate the bacteria Penicillium and Streptococcus. It provides a controlled environment for the growth and study of these microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Merck Group (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Sk mel 28, by Merck Group (2 mentions)
Roswell park memorial institute (rpmi), by Merck Group (2 mentions)
Sk mel 28, by American Type Culture Collection (2 mentions)
Roswell park memorial institute (rpmi), by American Type Culture Collection (2 mentions)
A375 human melanoma, by American Type Culture Collection (1 mentions)
Mda mb 231 human breast adenocarcinoma, by American Type Culture Collection (1 mentions)
A549 human lung adenocarcinoma, by American Type Culture Collection (1 mentions)
Hacat, by Merck Group (1 mentions)
Hacat, by American Type Culture Collection (1 mentions)
3 protocols using penicillin strep
1
Culturing Human Melanoma and Skin Cells
2
Culture of Human Melanoma Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human melanoma cell lines: A375, RPMI, and SK-MEL-28 were purchased from the American Type Culture Collection (ATCC). A375 were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) high-glucose medium supplemented with 10% fetal bovine serum (FBS, Gibco, ThermoFisher Scientific, Waltham, MA, USA) and 1% penicillin/Strep, 10,000 IU/mL (Sigma-Aldrich), and RPMI and SK-MEL-28 were cultured in Eagle’s Minimum Essential Medium (EMEM, Sigma-Aldrich) high-glucose medium supplemented with 10% fetal bovine serum (FBS, Gibco, ThermoFisher Scientific) and 1% penicillin/Strep, 10,000 IU/mL (Sigma-Aldrich). The cells were incubated under standard temperature conditions of 37 °C and humidity containing 5% CO2.
3
Cell Culture Conditions for Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A375 human melanoma, A549 human lung adenocarcinoma, and MDA-MB-231 human breast adenocarcinoma cell lines were purchased from the American Type Culture Collection (ATCC); HaCaT-human immortalized keratinocyte cell line was provided by the University of Debrecen, Hungary as a kind gift.
All the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) high-glucose medium supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/Strep, 10,000 IU/ml (Sigma-Aldrich; Merck KGaA). The cells were incubated under standard temperature conditions of 37°C and humidity containing 5% CO2.
All the cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) high-glucose medium supplemented with 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/Strep, 10,000 IU/ml (Sigma-Aldrich; Merck KGaA). The cells were incubated under standard temperature conditions of 37°C and humidity containing 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!