After being starved for 12 h, larvae were injected with 1 µL, 5 µL, 10 µL, and 15 µL of an aqueous solution (1 mg·mL−1) of Lucifer Yellow CH dipotassium salt (Fluka, λEx = 428 nm, λEm = 535 nm), with 4 replicates for each injection volume. As controls, 4 starved larvae, not-injected, were used. Larvae were then fed on lima bean plants for 5 min, wounding areas were pictured with a LEICA LMD6000 fluorescence microscope, and wounding sizes were measured as described above.
Lmd6000 fluorescence microscope
Manufactured by Leica
The LMD6000 is a fluorescence microscope designed for life science applications. It features high-resolution imaging and advanced optics to enable detailed analysis of biological samples. The LMD6000 is capable of capturing fluorescent signals with precision and clarity, supporting a range of research and laboratory workflows.
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Lab products found in correlation
2 protocols using lmd6000 fluorescence microscope
1
Lucifer Yellow Dye Injection in Larvae
2
Wounding Lima Bean Leaves with Insect Herbivores
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Lima bean leaves were treated by S. littoralis larva (injected with Lucifer Yellow solution, 5 µL, 1 mg·mL−1), SpitWorm (delivering a diluted (1:10) Lucifer Yellow solution (5 µL, 1 mg·mL−1) in 44 μL of filtered larval regurgitate at a flow rate of 10 nL·s−1), and by MecWorm and a razor blade, both without fluorescent labeling. Pictures of the wounding areas were taken with a LEICA LMD6000 fluorescence microscope.
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