Anti tigar sc 166290

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-TIGAR (sc-166290) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that targets the TIGAR (TP53-Induced Glycolysis and Apoptosis Regulator) protein. TIGAR is involved in regulating cellular metabolism and apoptosis. The core function of this antibody is to detect and bind to the TIGAR protein in various experimental and analytical applications.

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Lab products found in correlation

Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Cisplatin, by MedChemExpress (1 mentions) Affinipure goat anti rabbit igg h l sa00001 2, by Proteintech (1 mentions) Affinipure goat anti mouse igg h l sa00001 1, by Proteintech (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Anti β actin, by Proteintech (1 mentions) Anti parp 9532, by Cell Signaling Technology (1 mentions) Anti β actin sc 8432, by Santa Cruz Biotechnology (1 mentions) Ripa buffer, by Beyotime (1 mentions) Anti nrf2 16396 1 ap, by Proteintech (1 mentions) Anti tomm20 11802 1 ap, by Proteintech (1 mentions)

2 protocols using anti tigar sc 166290

Cisplatin Cytotoxicity Assay and Apoptosis Markers

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Cisplatin (MedChemExpress, New Jersey, USA). 3-(4,5-dimetrylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, St. Louis, MO, USA).
The following antibodies were used: anti-Bax(50599-2-Ig), anti-Bcl-2 (12789-1-AP), anti-Cytochrome c(10993-1-AP), anti-p53(10442-1-AP), anti-HK2(22029-1-AP), anti-VDAC1(55259-1-AP), anti-β-actin(66009-1-Ig), peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) (SA00001-1), peroxidase- conjugated AffiniPure goat anti-rabbit IgG (H + L) (SA00001-2) (Proteintech, Chicago, IL, USA); anti-G6PD (sc-373886) and anti-TIGAR (sc-166290) (Santa Cruz, CA, USA).

Fu J., Yu S., Zhao X., Zhang C., Shen L., Liu Y, & Yu H. (2022). Inhibition of TIGAR Increases Exogenous p53 and Cisplatin Combination Sensitivity in Lung Cancer Cells by Regulating Glycolytic Flux. International Journal of Molecular Sciences, 23(24), 16034.

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Protein Analysis of Mitochondrial Regulators

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Whole‐cell lysis was prepared with RIPA buffer (Beyotime, P0013B) and centrifuged at 12,000 g for 15 minutes. Protein concentrations were measured using Pierce® BCA protein assay kit (Thermo Scientific, 23225). Immunoblot was performed using specific primary antibodies and fluorescein‐conjugated secondary goat anti‐rabbit/mouse antibody (Gene, 926‐32211) and then detected using Odyssey fluorescence scanner.
Anti‐Parkin (4211), anti‐Ub (3936), anti‐p‐Ub (Ser65) (62802), and anti‐PARP (9532) were purchased from Cell Signaling Technology. Anti‐gankyrin (sc‐101498), anti‐BNIP3 (sc‐56167), anti‐β‐actin (sc‐8432), and anti‐TIGAR (sc‐166290) were purchased from Santa Cruz Biotechnology, Inc. Anti‐G6PD (A‐11234), anti‐PGD (A‐0563), anti‐TKT (A‐13553), anti‐FUNDC1 (A‐16318), anti‐HSP60 (A‐0564), and anti‐TALDO (A‐13551) were purchased from Abclonal. Anti‐Nrf2 (16396‐1‐AP) and anti‐TOMM20 (11802‐1‐AP) were purchased from Proteintech Inc.

Yang C., Cui X., Ding Z., Jiang T., Feng X., Pan Y., Lin Y., Shang T., Wang Q., Pan J., Wang J., Wang H, & Dong L. (2022). Gankyrin and TIGAR cooperatively accelerate glucose metabolism toward the PPP and TCA cycle in hepatocellular carcinoma. Cancer Science, 113(12), 4151-4164.

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