4′,6-Diamidino-2-phenyl-indole (DAPI) (C0065) was purchased from Solarbio (Beijing, China). ChromoTek GFP-Trap (gta) and DYKDDDDK Fab-Trap (ffa) were purchased from Proteintech (Wuhan, China). BeaverBeads GSH (70601-K10) and His tag (70501-K10) were purchased from Beaverbio (Suzhou, China). TRIzol reagent (15596018), Lipofectamine 2000 (11668019), and Lipofectamine RNAiMAX (13778150) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A2ti-1 (HY-136465) and PP2 (HY-13805) were purchased from MedChemExpress (South Brunswick, NJ, USA).
Pp2 hy 13805
Manufactured by MedChemExpress
Sourced in United States
PP2 (HY-13805) is a potent and selective inhibitor of Src family tyrosine kinases, with an IC50 of 4 nM for Src. It functions by blocking the ATP-binding site of Src, thereby inhibiting its enzymatic activity.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole dapi c0065, by Solarbio (1 mentions)
Chromotek gfp trap, by Proteintech (1 mentions)
Hela cell, by American Type Culture Collection (1 mentions)
Mg132 hy 13259, by MedChemExpress (1 mentions)
Trichostatin a hy 15144, by MedChemExpress (1 mentions)
Beas 2b cell line, by American Type Culture Collection (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Keygen Biotech (1 mentions)
Puromycin, by Solarbio (1 mentions)
Penicillin and streptomycin, by Solarbio (1 mentions)
2 protocols using pp2 hy 13805
1
DAPI-Based Cellular Labeling Protocol
2
Evaluating DNA Repair Mechanisms in Cell Lines
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The human cervical cancer cell line HeLa cells, human embryonic kidney cell line HEK293T, and BEAS-2B cell line were purchased from ATCC; U2OS cells containing NHEJ reporter system and HRR reporter system, and pCAGGS-I-SceI plasmid were gifts from Liu Songbai's group at Suzhou Vocational Health College. HK2 cell line was gift from Xu Qian's group at Nanjing Medical University. These cells were cultured in Dulbecco's modified eagle's (DMEM) or RPMI-1640 (Keygen Biotech, China) supplemented with 10% fetal bovine serum (Biochannel, China) and 1% penicillin and streptomycin (Solarbio, Beijing, China). For stable knockdown GAPDH HeLa cells, the cell was infected with specific lentivirus vectors for 48 h and then selected with puromycin (Solarbio, China) for 3 weeks. Cycloheximide (S7418), camptothecin (S1228), adriamycin (S1208), and etoposide (S1225) were purchased from Selleckche. MG132 (HY-13259), Trichostatin A (HY-15144), and PP2 (HY-13805) were obtained from MedChem Express. Heptelidic acid (GC43816) was purchased from GLPBIO. The recombinant HDAC1 (IICH8936) and Maspin (ICH8931) were purchased from ImmunoClone.
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