To analyze the growth rate of HT-29 after exposure to RGZ, (R,S)-3 , (S)-3 , (R,S)-7 , cells were plated in 24-well plates at density of 50000 cells/cm2. After treatment, the cells were washed with PBS, trypsinized and collected in culture medium. Cells were counted by means of a Burker’s hemocytometer and by automated cell counter (Roche Applied, Penzberg, Germany)60 (link).
Automated cell counter
Manufactured by Roche
Sourced in Germany
The Automated Cell Counter is a laboratory instrument used for the quantitative analysis of cells. It provides an automated and accurate method for counting and measuring various cell types in a sample. The device utilizes advanced optical technologies to detect and enumerate cells, offering a reliable and efficient way to obtain cell counts for research, diagnostic, or quality control purposes.
Automatically generated - may contain errors
Lab products found in correlation
Hemocytometer, by Roche (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
Cck 8, by Merck Group (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
T175 flask, by Corning (1 mentions)
Trypanblue, by Roche (1 mentions)
3 protocols using automated cell counter
1
Growth rate of HT-29 cells with RGZ
2
Cell Viability Assay with CCK-8
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After corresponding treatments, HT22 cells were suspended until homogeneous distribution, and counted by an automated cell counter (Roche Diagnostics, Mannheim, Germany). 100 μL of cell suspension per well was seeded into a 96-well plateat a density of 3 × 104 cells/mL. Cell viability was detected by the Cell Counting Kit-8 (CCK-8; Sigma-Aldrich) in light of the manufacturer’s protocol. Briefly, 10 μL of CCK-8 solution was added into each well of the 96-well plate at 0, 6, 12, 24, and 48 h. After two h of culture at 37 ℃ in a humidified atmosphere of 5% CO2, optical density (OD) values were detected using a plate reader at 450 nm (Sigma-Aldrich). The cell growth curves were plotted on the basis of the OD values.
3
Vero Cell Line Maintenance Protocol
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The African green monkey kidney Vero cell line
was sourced from internal cell banks, grown and maintained in T175
flasks (Corning) cultured with Dulbecco’s modified Eagle media
(DMEM)/high glucose (Gibco) supplemented with 10% fetal bovine serum
(FBS) (ATCC) and 1% Pen-Strep (Gibco) in a >90% relative humidified
incubator at 37 °C with 5% pCO2.
Cells were dissociated using 0.25% Trypsin-EDTA for 4 ± 2 min
at 37 °C, 5% pCO2, and >90% rH
and
quenched with DMEM/high glucose (10% FBS, 1% Pen-Strep). Dissociated
cells from multiple flasks were pooled and resuspended in DMEM/high
glucose (10% FBS, 1% Pen-Strep). Cell viability was determined before
seeding or passaging with a Cedex automated cell counter and trypan
blue (Roche).
was sourced from internal cell banks, grown and maintained in T175
flasks (Corning) cultured with Dulbecco’s modified Eagle media
(DMEM)/high glucose (Gibco) supplemented with 10% fetal bovine serum
(FBS) (ATCC) and 1% Pen-Strep (Gibco) in a >90% relative humidified
incubator at 37 °C with 5% pCO2.
Cells were dissociated using 0.25% Trypsin-EDTA for 4 ± 2 min
at 37 °C, 5% pCO2, and >90% rH
and
quenched with DMEM/high glucose (10% FBS, 1% Pen-Strep). Dissociated
cells from multiple flasks were pooled and resuspended in DMEM/high
glucose (10% FBS, 1% Pen-Strep). Cell viability was determined before
seeding or passaging with a Cedex automated cell counter and trypan
blue (Roche).
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