RPMI-1640 medium, fetal bovine serum (FBS) and penicillin were purchased from Invitrogen; Thermo Fisher Scientific. Antibodies against TPD52, transforming acidic coiled-coil-containing protein 3 (TACC3), Ephrin A1(EFNA1), transferrin (TRF) and β-actin were obtained from Abcam. DNAJB1, LDH, trypsin and goat anti-rabbit/mouse secondary antibodies conjugated to horseradish peroxidase were from Sigma-Aldrich. The Total RNA extraction kit, SYBR-Green detection system and the Bradford protein assay kit were purchased from Tiangen Biotech. The ultrafiltration device, ECL reagent and polyvinylidene difluoride (PVDF) were from Millipore. The DAB detection kit was from Maixin Biotech. The iTRAQ labeling Reagents kit was from Applied Biosystems.
Tacc3
Manufactured by Abcam
Sourced in United Kingdom
TACC3 is a protein that functions as a subunit of the HAUS augmin-like complex, which is involved in the regulation of microtubule organization during cell division. The TACC3 protein contributes to the stabilization and organization of microtubules at the centrosome, which is essential for proper chromosome segregation during mitosis.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride pvdf, by Merck Group (1 mentions)
Dab detection kit, by Maixin Group (1 mentions)
Sybr green detection system, by Tiangen Biotech (1 mentions)
Ecl reagent, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
β actin, by Abcam (1 mentions)
Total rna extraction kit, by Tiangen Biotech (1 mentions)
Bradford protein assay kit, by Tiangen Biotech (1 mentions)
β actin, by Merck Group (1 mentions)
2 protocols using tacc3
1
Proteomic and Transcriptomic Analysis
2
Western Blot Analysis of Signaling Proteins
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Cell lysates were generated from cells mock-treated or treated with AZD2858 at 0, 0.1, 1, and 10 µM. The lysates were prepared for SDS-PAGE electrophoresis as previously described [22 (link)]. Separated proteins were probed with the following antibodies in Western blots as previously described [22 (link)]. Antibodies used included anti-beta-catenin (1/1000, #9562, CST, Cambridge, UK), phospho-beta-catenin (1/1000, #9561, CST, Cambridge, UK), GSK-3 alpha (1/1000, #9338, CST, Cambridge, UK), GSK-3 beta (1/1000, #9315, CST, Cambridge, UK), TACC3 (1/500, #ab56595, Abcam, Cambridge, UK), Akt (1/1000, #9272, CST), phospho-Akt (1/1000, #9271, CST, Cambridge, UK), and beta-actin (1/10,000, #A1978, Sigma, Poole, UK).
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