The animal experiment was performed with the approval of the Animal Experimental Committee (Reference No. 212024) of Tokai University School of Medicine. Mouse malignant melanoma cells (B16-F10) were cultured with 10% FBS-DMEM (High Glucose) (FUJIFILM Wako Pure Chemical Co. Ltd., Osaka, Japan) medium added to L-glutamine and penicillin-streptomycin in a 25 cm2 flask (BM Equipment Co. Ltd., Tokyo, Japan) at 37 °C in 5% CO2 incubator. B16-F10 cells cultured with 80% confluency were harvested with 0.05% trypsin-EDTA (Thermo Fisher Scientific Co. Ltd., Waltham, MA, USA) and suspended at 5 × 105 cells/100 μL of DMEM alone after washing with PBS. Cell suspension (100 µL) was injected into the right flank of 8-week-old male C57BL/6J mice (The Jackson Laboratory Japan Inc., Yokohama, Japan), and an equal volume of DMEM was injected into the corresponding site of healthy mice.
Fbs dmem high glucose
Manufactured by Fujifilm
Sourced in Japan
FBS-DMEM (High Glucose) is a cell culture media formulation that contains Dulbecco's Modified Eagle's Medium (DMEM) with a high glucose concentration, supplemented with Fetal Bovine Serum (FBS). This product is designed to support the growth and maintenance of a variety of cell types in in vitro cell culture applications.
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2 protocols using fbs dmem high glucose
1
Murine Melanoma Xenograft Model
2
Osteogenic Differentiation Protocols for Cell Lines
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MC3T3-E1 cells and ATDC5 cells were obtained from RIKEN Cell Bank. MC3T3-E1 cells were maintained in 10% fetal bovine serum (FBS)/alpha-MEM (A10490-01; Life Technologies) supplemented with 1% penicillin/streptomycin. ATDC5 cells were maintained in 5% FBS/Dulbecco's Modified Eagle Medium (DMEM)-F12 (11039–021; Thermo Fisher) supplemented with 1% penicillin/streptomycin. Mouse mesenchymal stromal cells (MSCs) and calvarial osteoblasts were isolated as previously described [10 (link)]. Human MSCs were obtained from LONZA. Mouse and Human MSCs were maintained in 10% FBS/DMEM high glucose (043–30085; Wako) supplemented with 1% penicillin/streptomycin. Osteogenic induction was performed by culturing cells with 10% FBS/DMEM high glucose supplemented with 0.1 μM dexamethasone, 10 mM β-glycerophosphate, and 50 μg/ml ascorbic acid phosphate (osteogenic medium). For osteogenic induction of hMSCs, 10 ng/ml recombinant human bone morphogenetic protein (BMP)2 (rhBMP2: 355-BM; R&D) was added to the osteogenic medium. Von Kossa staining and alizarin red staining were performed as previously described [11 ].
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